Tissue Disaggregation Device with Heat Transfer Plate
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Solution Overview
Problem
Existing methods for tissue disaggregation do not adequately consider thermal control during the process, which affects the viability and recovery of cellular material, particularly during freezing and thawing.
Innovation Solution
A treading device with a heat transfer plate and a disposable flexible container for tissue disaggregation, which allows for controlled temperature management during the process, ensuring improved thermal control and cell viability by using a motor-driven treading mechanism within a controlled temperature environment.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If tissue disaggregation is performed without adequate thermal control, then the disaggregation process can proceed, but cell viability and recovery are reduced
Solution Approach 1:
A heat transfer plate is introduced as an intermediary component between the tissue sample and the thermal environment. The plate facilitates controlled heat transfer during disaggregation and freezing processes, mediating the thermal interaction to preserve cell viability while enabling effective tissue processing.
Solution Approach 2:
The system dynamically changes temperature parameters during the disaggregation process. By controlling the thermal state through the heat transfer plate, the method optimizes conditions for enzymatic digestion, mechanical disaggregation, and subsequent freezing, thereby improving cell recovery and viability at each stage.
2Reliability
If a closed system is used for tissue disaggregation, then sterility is maintained, but thermal management becomes more challenging
Solution Approach 1:
The heat transfer plate serves multiple functions within the closed system: it enables thermal control during disaggregation, facilitates uniform freezing of the tissue sample, and maintains the integrity of the closed sterile environment throughout the process. This multi-functionality resolves the conflict between maintaining sterility and achieving effective thermal management.
3Reliability
If freezing is performed without controlled thermal conditions, then the process is simpler, but cell damage increases
Solution Approach 1:
The heat transfer plate is positioned and prepared in advance before freezing begins. This preliminary arrangement ensures that thermal control is immediately effective when freezing starts, preventing thermal shock and ice crystal formation that would damage cells. The plate is pre-configured to facilitate gradual, controlled freezing that preserves cell integrity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution enhances the efficiency and effectiveness of tissue disaggregation by maintaining optimal temperature conditions, improving cell viability and recovery, and allowing for seamless processing from disaggregation to cryopreservation and thawing within a closed system.
Implementation Method 1
a heat transfer plate (150) for transferring heat energy to or from the sample receiving area (148)
Implementation Method 2
A treading device for the disaggregation of tissue into individual cells or cell clumps
Data Source
AI summary
Disclosed is a device (100, 200) for the disaggregation of tissue samples into individual cells or cell clumps in a closed flexible tissue sample bag (10); the device including two or more resilient feet (134/136, 234/236) which tread sequentially a tissue sample bag receiving area (148,248). Also disclosed is a heat transfer plate (150, 250) for transferring heat energy to or from the area (148,248), the plate having one plate surface (151,251) adjacent the area (148,248) and an opposing surface (152,252) exposed to external thermal influence which faces away from the area (148,248). Further disclosed is a tissue sample receiving bag (10) comprising one or more flexible plastics cavity (12) formed from two layers of the plastics sealed around their edges to form a generally rectilinear periphery with the cavity or cavities (12) within the periphery, and at one side of the periphery is formed one or more sealable access ports (16). One part of the bag is left unsealed to provide a tissue sample receiving opening.