Biological Tissue Drying with Pressure Compensation for Transparency
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing methods for producing biodegradable biological tissue face challenges such as non-transparency, stiffness due to pressure peaks, and insufficient water exchange, leading to non-biodegradability and hindered substance access.
Innovation Solution
A method involving stabilization with glycerol and polyethylene glycol followed by controlled drying between permeable fabric layers and pressure compensation layers to maintain tissue flexibility and transparency, allowing for biodegradability and mechanical stability.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If decellularized tissue is used as a scaffold, then biocompatibility and structural integrity are improved, but residual cellular material and DNA cause immunogenicity and inflammation
Solution Approach 1:
The patent employs strong oxidizing agents including sodium hydroxide (NaOH) at concentrations of 0.1-10% for 1-72 hours, hydrogen peroxide (H2O2) at 0.1-10% for 1-48 hours, and ozone treatment at 1-100 mg/L for 1-48 hours. These oxidants effectively remove residual cellular material and DNA from the decellularized tissue scaffold, reducing immunogenicity while preserving the extracellular matrix structure and biocompatibility
Solution Approach 2:
The patent uses combination treatments involving multiple decellularization and sterilization methods. For example, combining mechanical disruption with chemical oxidation (NaOH/H2O2), or combining ozone treatment with UV irradiation. These composite approaches ensure complete removal of immunogenic materials while maintaining scaffold integrity and biological functionality
2Loss of substance
If conventional decellularization methods are used, then cellular material is removed, but the process is time-consuming and lacks standardization
Solution Approach 1:
The patent establishes predetermined standardized protocols for decellularization with specific parameters: NaOH concentration (0.1-10%) and time (1-72 hours), H2O2 concentration (0.1-10%) and time (1-48 hours), ozone concentration (1-100 mg/L) and time (1-48 hours). These pre-established protocols eliminate trial-and-error processes and provide reproducible, time-efficient decellularization procedures
Solution Approach 2:
The patent systematically optimizes and standardizes critical parameters including chemical concentration, treatment duration, temperature, and pH levels. By establishing specific parameter ranges (e.g., NaOH 0.1-10% for 1-72 hours, ozone 1-100 mg/L for 1-48 hours), the method achieves consistent cellular material removal within controlled timeframes, reducing processing variability and overall time
3Object-affected harmful factors
If extensive decellularization treatment is applied, then immunogenicity is reduced, but structural integrity and mechanical properties deteriorate
Solution Approach 1:
The patent applies controlled oxidation treatments with specific concentration and time parameters to achieve sufficient cellular material removal without excessive damage to the extracellular matrix. For example, using NaOH at 0.1-10% for 1-72 hours or H2O2 at 0.1-10% for 1-48 hours provides adequate decellularization while preserving scaffold strength, avoiding the need for prolonged or high-concentration treatments that would compromise structural integrity
Solution Approach 2:
The patent employs gentle initial decellularization steps followed by milder finishing treatments. The sequence of treatments (e.g., mechanical disruption first, then controlled chemical oxidation) progressively removes cellular material while preserving the underlying matrix structure. This staged approach cushions the scaffold against excessive mechanical or chemical stress that would compromise structural integrity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Results in a biodegradable, transparent, and mechanically stable tissue suitable for medical implants, enabling cellular ingrowth and optical clarity during operations.
Implementation Method 1
ozone at a concentration of 1 to 100 mg/L for a period of 1 to 48 hours
Implementation Method 2
hydrogen peroxide for a period of 1 to 48 hours
Implementation Method 3
hydrogen peroxide for a period of 1 to 48 hours
Implementation Method 4
sodium hydroxide for a period of 1 to 72 hours
Data Source
Figure 1~2
Figure 3
Figure 4A~4F
AI summary
A first aspect of invention relates a method for preparing a biodegradable biological tissue, preferably a collagen containing tissue. The (decellularized) biological tissue is first stabilized by a tissue water replacing material and then dried while applying an elevated pressure to the biological tissue. A second aspect of the invention relates to a method for producing a non-biodegradable biological tissue tube (i.e. hollow cylinder), preferably a collagen containing tissue tube.