Tissue Embedding Array for Multiplexed Sectioning Quality
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Solution Overview
Problem
Existing tissue processing and analysis methods are time-consuming, costly, and not practical for multiplexing complex or heterogeneous specimens, particularly when dealing with biopsy punch tissue grids, which require frequent thawing and limit sectioning quality and speed.
Innovation Solution
A method involving a carrier with recesses for specimens, filled with an embedding compound that is solidified or frozen, creating an array of fastened specimens in a block, allowing for efficient multiplexing and cost-effective analysis without repeated thawing and freezing cycles.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If biopsy punch tissue grids are used for multiplexing, then multiple specimens can be processed simultaneously, but frequent thawing is required which limits sectioning quality and speed
Solution Approach 1:
Multiple individual tissue specimens are merged into a single solidified embedding block containing all specimens in array form. This allows simultaneous sectioning of multiple specimens through the single block without repeated thawing, combining the benefits of multiplexing with maintained sectioning quality.
Solution Approach 2:
Tissue specimens are pre-positioned in recesses of a carrier and pre-solidified in embedding compound before final array formation. This preliminary arrangement ensures proper positioning and prevents displacement during subsequent processing steps.
2Ease of manufacture
If specimens are placed in holes of frozen embedding compound, then tissue array can be generated, but the process is time consuming and not practical for complex specimens
Solution Approach 1:
The carrier with recesses is prepared in advance, and specimens are pre-positioned in the recesses before adding embedding compound. This preliminary arrangement eliminates the need for time-consuming post-positioning adjustments and facilitates efficient array generation.
Solution Approach 2:
The recesses in the carrier automatically hold specimens in correct positions during embedding. The structure itself provides the positioning function, eliminating the need for additional positioning steps or tools.
3Adaptability or versatility
If frozen embedding compound with holes is used, then tissue specimens can be arrayed, but sectioning requires frequent thawing which reduces speed
Solution Approach 1:
Multiple specimens are combined into a single solidified block structure, allowing the sectioning apparatus to process all specimens simultaneously through one continuous sectioning operation, eliminating repeated thawing cycles.
Solution Approach 2:
The embedding compound is solidified to a stable state that maintains specimen positions without requiring frequent phase changes. This parameter change from frozen to solidified state eliminates the need for repeated thawing while preserving array configuration.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables rapid and cost-effective multiplexed tissue processing by preventing specimen displacement and preserving tissue quality, facilitating simultaneous sectioning and analysis of multiple specimens without the need for repeated thawing and freezing.
Implementation Method 1
solidifying or freezing the embedding compound and the specimens, thereby generating an array of frozen specimens in a block of frozen embedding compound
Data Source
Figure 1A~1C
Figure 2A~2E
Figure 3A~3P
AI summary
The present invention provides a method for generating an array of fastened specimens comprising the steps of providing a carrier with a bottom surface that comprises an array of at least two recesses, placing at least two specimens on the carrier at the recesses, filling the carrier at least in the space between the specimens with a embedding compound, solidifying the embedding compound and fastening the specimens in the embedding compound, thereby generating an array of fastened specimens in a block of solidified embedding compound. The invention further provides a kit with means for such a method.