Anti-Tissue Factor Affinity Resin for Selective Protein Purification
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Solution Overview
Problem
Existing methods for purifying tissue factor-containing proteins are inefficient and lack specificity, particularly in isolating and purifying tissue factor from complex biological samples.
Innovation Solution
Development of affinity chromatography resins using anti-tissue factor antibodies or antigen-binding fragments attached to a base resin, specifically utilizing CDRs from heavy and light chain variable domains, to enhance the purification process.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional chromatography resins are used, then basic purification function is provided, but bacterial contamination occurs and purification capacity is insufficient
Solution Approach 1:
The patent applies composite materials by combining conventional chromatography resin with silver ions to create a new composite chromatography resin. The silver ions are incorporated into the resin structure, creating a material that maintains the original purification function while adding antibacterial properties. This composite approach resolves the contradiction by integrating two functional components into a single material system.
Solution Approach 2:
The patent changes the chemical composition parameter of the chromatography resin by introducing silver ions. This parameter change transforms the resin from a purely chromatographic material to one with dual functionality: chromatographic purification and antibacterial action. The silver ion concentration and distribution are controlled to achieve optimal both purification and antibacterial effects.
2Productivity
If conventional chromatography resins are used, then standard purification is achieved, but purification capacity is insufficient for high-value products
Solution Approach 1:
The patent applies local quality by creating regions within the chromatography resin that have enhanced purification capacity through silver ion incorporation. The silver ions are distributed throughout the resin structure, creating localized zones with improved binding and purification capabilities. This allows different regions of the resin to contribute differently to the overall purification process, enhancing both capacity and selectivity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The affinity chromatography resins provide high specificity and efficiency in purifying tissue factor-containing proteins, enabling effective isolation and purification from biological samples.
Implementation Method 1
silver ions have an antibacterial effect
Implementation Method 2
chromatography resin
Data Source
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AI summary
An affinity chromatography resin comprising an anti-tissue factor antibody or antigen-binding fragment thereof attached to a base resin, and methods of using the same.