Sealed Tissue Imaging Chamber with Segmented Cavities
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Solution Overview
Problem
Current methods for observing living tissues at the micro level are limited by the need for two-dimensional techniques and lack of appropriate chambers for transparent large sample imaging, leading to tissue damage and high costs.
Innovation Solution
A chamber apparatus composed of cover slips with interposed cavities and outer walls that can be easily coupled to prevent leakage and bubble formation, allowing for the observation of large living tissues using a tissue transparency technique.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional 2D imaging techniques with tissue sectioning are used, then micro-level analysis is achieved, but tissue damage occurs and the process is complex
Solution Approach 1:
The chamber is divided into multiple compartments (first chamber, second chamber, third chamber) with separate cavities for different functions: sample placement, mounting solution storage, and bubble removal. This segmentation allows each compartment to perform its specific function independently, enabling complete tissue imaging without physical sectioning that would cause damage.
Solution Approach 2:
A mounting solution is introduced as an intermediary substance between the tissue sample and the imaging system. This solution replaces air gaps and enables light transmission through the entire tissue thickness, allowing 3D imaging without physical sectioning. The mounting solution chamber stores and delivers this intermediary substance to the sample.
2Measurement precision
If tissue transparency technique is used for large sample imaging, then complete tissue imaging is achieved, but leakage and bubble formation occur
Solution Approach 1:
The chamber is divided into multiple compartments (first chamber, second chamber, third chamber) with separate cavities for different functions: sample placement, mounting solution storage, and bubble removal. This segmentation allows each compartment to perform its specific function independently, enabling complete tissue imaging without physical sectioning that would cause damage.
Solution Approach 2:
Each chamber compartment has specific local properties optimized for its function: the first chamber has a cavity for sample placement with specific volume, the second chamber stores mounting solution, and the third chamber removes bubbles. The outer walls are coupled with sealing structures at specific locations to prevent leakage while allowing controlled access.
3Measurement precision
If conventional mounting methods are used, then tissue observation is achieved, but the process is complex and expensive
Solution Approach 1:
The chamber serves multiple functions within a single integrated structure: it holds the tissue sample, stores mounting solution, removes bubbles, and provides a sealed imaging environment. The outer walls can be coupled and decoupled for easy assembly and cleaning, making the single chamber suitable for multiple imaging sessions and different samples.
Solution Approach 2:
The chamber design enables self-contained operation where the mounting solution is stored within the chamber itself rather than requiring external reservoirs. The bubble removal chamber provides automatic bubble elimination, and the sealed structure maintains the mounting solution environment without continuous external intervention.
Data Source
AI summary
An apparatus for observing image of living tissue having a first cover slip and a second cover slip; and one or more chambers interposed between the first cover slip and the second cover slip and having a cavity therein; in order to observe a living tissue loaded in the cavity of the chamber above is provided.The apparatus for observing image of living tissue of the present invention can be easily prepared with a simple process and a low cost, and facilitates the observation of images of living tissues on both sides, so that an entire image of a huge living tissue at the level of several hundred micrometers can be obtained by using a tissue transparency technique, which is a distinguished advantage of the apparatus of the invention. In the apparatus of the invention, the outer wall part forming the chamber is strongly bound so that the leakage of the mounting solution and the bubble generation can be prevented, indicating the damage of the living tissue can also be prevented.