Animal Tissue Matrix Decellularization Preserving Biomechanical Strength
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Solution Overview
Problem
Current methods for manufacturing animal decellularized tissue matrix materials are complex and vary in efficiency, leading to differences in clinical performance due to changes in biochemical composition and biomechanical properties during the processing steps, which affect host response and regeneration outcomes.
Innovation Solution
A method involving specific steps such as low-temperature preservation, gentle rehydration, moderate alkaline disinfection, enzymatic decellularization, and controlled terminal sterilization to maintain the integrity and biomechanical strength of the tissue matrix, while removing immunogenic antigens and viruses, is employed to produce a high-quality acellular tissue matrix material.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If physical treatment and chemical treatment are combined for decellularization, then cell removal efficiency is improved, but damage to the tissue matrix structure and biomechanical properties increases
Solution Approach 1:
The decellularization process is divided into distinct sequential stages: physical treatment stage (ultrasonic wave treatment) and chemical treatment stage (detergent treatment). Each stage is optimized independently with controlled parameters (ultrasonic power, treatment time, detergent concentration, temperature) to achieve cumulative cell removal while minimizing cumulative damage to the extracellular matrix structure and biomechanical properties
Solution Approach 2:
The patent optimizes specific parameters for each treatment stage including ultrasonic wave power (20-100W), treatment time (5-60 minutes), detergent concentration (0.1-1% v/v), and temperature (4-37°C). By precisely controlling these parameters, the process achieves effective decellularization while preserving the integrity and biomechanical strength of the tissue matrix
2Ease of operation
If enzymatic treatment is used to alter density and porosity, then cell surface connection is improved, but the tissue extracellular matrix structure is damaged
Solution Approach 1:
Enzymatic treatment with collagenase or trypsin is applied as a preliminary step before detergent-based decellularization. This preliminary enzymatic action selectively degrades cell surface connections and facilitates subsequent cell removal, while the enzyme concentration and treatment time are controlled to prevent excessive degradation of the extracellular matrix structure
Solution Approach 2:
The patent controls enzymatic treatment parameters including enzyme concentration (0.01-0.1% w/v), treatment time (30-120 minutes), and temperature (37°C) to achieve optimal cell surface connection alteration while minimizing damage to the tissue extracellular matrix structure and maintaining its stability
3Reliability
If multiple processing steps are performed for decellularization, then antigenicity reduction is improved, but variation in biochemical composition and ultrastructure increases
Solution Approach 1:
The processing protocol is segmented into standardized sequential steps: physical treatment, enzymatic treatment, chemical treatment, and washing stages. Each step has defined parameters and duration, ensuring consistent antigen removal while maintaining uniform biochemical composition and ultrastructure across different batches of tissue matrix materials
Solution Approach 2:
The patent implements continuous washing steps between each treatment stage to remove residual chemicals and cellular debris. This continuous action ensures complete antigen removal while preventing accumulation of treatment byproducts that could cause variation in biochemical composition, thereby maintaining consistency in the final tissue matrix product
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method effectively preserves the biomechanical properties and removes immunogenic antigens, enhancing the flexibility and integration performance of the tissue matrix, ensuring safe and effective tissue regeneration without significant damage to the matrix structure.
Implementation Method 1
The cellular membrane is damaged by stirring or ultrasonic, mechanical massage or pressurization
Implementation Method 2
The cellular membrane is damaged by stirring or ultrasonic
Implementation Method 3
Enzymatic treatment method, such as the use of trypsin, can alter the density and porosity of the tissue extracellular matrix, and cut the connection between the cell surface and the tissue extracellular matrix
Implementation Method 4
The matrix of the tissue and organ is a three-dimensional scaffold composed of various complex structural proteins and functional proteins
Implementation Method 5
further facilitating the subsequent decellularization and washing using a chemical detergent
Data Source
AI summary
A method for manufacturing an animal acellular tissue matrix material and a tissue matrix material manufactured by the same. The tissue matrix material manufactured by the method retains an original basic scaffold structure of a tissue extracellular matrix, with an antigen causing immunological rejection in a human body being effectively removed from the animal tissue. An animal dermal matrix manufactured by the method retains the biological integrity of a natural dermal tissue matrix and can be used for restoration and repair of lesion and missing tissues.