Acellular Tissue Perforation for Cell Penetration
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Solution Overview
Problem
Current methods for preparing acellular organic tissues for transplantation face challenges in achieving deep and uniform cell penetration, particularly in thicker tissues, leading to incomplete revitalization and limited transplant success.
Innovation Solution
The method involves creating a reticular structure of intersecting holes and optional reservoir cavities within the tissue, made using needles with controlled electric current, to facilitate rapid and uniform cell distribution and penetration throughout the tissue thickness.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If acellular organic tissue is prepared for transplantation using conventional methods, then the tissue structure is preserved, but cell penetration is insufficient in thicker tissues leading to incomplete revitalization
Solution Approach 1:
The tissue is segmented by creating a network of holes that divide the thick tissue into smaller compartments, allowing cells to penetrate more effectively throughout the entire tissue volume rather than attempting to penetrate a solid thick structure
Solution Approach 2:
The invention transforms the tissue into a porous structure by creating interconnected holes, enabling cell migration and penetration throughout the tissue matrix, which directly addresses the cell penetration problem in thicker tissues
2Length of stationary object
If the tissue thickness is increased to meet transplant requirements, then the tissue can replace larger organ defects, but cell penetration time increases and revitalization becomes incomplete
Solution Approach 1:
The invention adds a dimensional aspect by creating three-dimensional interconnected hole networks throughout the tissue thickness, transforming cell penetration from a surface-level two-dimensional process into a three-dimensional pathway system that reaches deep into thick tissues efficiently
3Stability of the object's composition
If conventional acellular preparation methods are used, then the connective tissue fibres are preserved, but the tissue lacks sufficient porosity for effective cell migration
Solution Approach 1:
The invention applies local quality changes by creating holes at specific locations and densities within the tissue, providing increased porosity where needed for cell migration while preserving the overall integrity and structure of the connective tissue fibres in the surrounding areas
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables complete and quick revitalization of thicker tissues, reducing treatment time and minimizing the risk of transplant failure by ensuring cells can reach and penetrate all parts of the tissue effectively.
Implementation Method 1
a plurality of holes (4) extending towards the inside of the tissue, characterised in that said plurality of holes (4) is made by means of one or more needles (2)
Data Source
Figure 1~3
AI summary
The invention concerns a method for the preparation of an acellular organic tissue of human or animal origin for revitalization, in particular for the introduction of living cells, comprising a stage in which the acellular organic tissue (2; 12) is provided with a plurality of holes (4; 14) made through its surface (8; 18) and extending towards the inside of the tissue (2; 12), wherein the plurality of holes (4; 14) is made by means of one or more needles. The holes (4; 14) intersect partially, thus forming holes (4; 14) partially communicating with each other. The invention also concerns a corresponding acellular organic tissue (2; 12) of human or animal origin prepared for revitalization, in particular for the introduction of living cells, comprising holes (4; 14) that are punctures made with needles and extending from the surface of the tissue (8; 18) towards its inside. The holes (4; 14) intersect at least partially and are therefore holes (4; 14) partially communicating with each other.