Tissue Preservation Gel Formulation for Safe Long-Term Storage
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Solution Overview
Problem
Existing tissue preservation methods are toxic, flammable, carcinogenic, and non-biodegradable, making them unsafe for handling and unsuitable for long-term preservation without tissue degradation, which hinders visual micro-analysis.
Innovation Solution
A non-toxic, biodegradable, thermally stable preservation solution with specific viscosity and impedance, formulated with Carbopol, triethanolamine, isopropyl alcohol, glycerin, and Nipagin, allowing for indefinite preservation and analysis via diagnostic imaging, involving histological fixation, dehydration, cutting, and staining processes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Duration of action of stationary object
If formaldehyde-based preservation products are used, then tissue preservation is achieved, but the solution becomes toxic, carcinogenic, and harmful to tissue quality for visual analysis
Solution Approach 1:
The patent changes the chemical composition parameters of the preservation solution by replacing formaldehyde with a combination of isopropyl alcohol (65-95%), glycerin (5-20%), and water (5-30%). This parameter substitution eliminates toxicity and carcinogenicity while maintaining preservation effectiveness and tissue quality for visual analysis.
2Duration of action of stationary object
If traditional preservation formulas are used, then tissue preservation is achieved, but the solutions are flammable and non-biodegradable
Solution Approach 1:
The patent creates a composite preservation solution combining isopropyl alcohol, glycerin, and water in specific proportions. This composite formulation achieves indefinite tissue preservation while being biodegradable and reducing flammability risks compared to traditional pure alcohol or formaldehyde solutions.
3Ease of operation
If preservation solutions with inadequate viscosity are used, then ease of application is improved, but tissue degradation occurs preventing long-term preservation
Solution Approach 1:
The patent optimizes the viscosity parameter of the preservation solution by adjusting the glycerin content (5-20%) and alcohol concentration (65-95%). This parameter optimization provides adequate viscosity for tissue immersion and long-term preservation while maintaining ease of application and pouring.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution enables safe, long-term preservation and analysis of biological tissues without significant degradation, ensuring non-toxicity, biodegradability, and thermal stability, while being cost-effective and suitable for diagnostic imaging techniques like microscopy and MRI.
Implementation Method 1
The solution has a viscosity and impedance calculated to allow the biological samples to be analyzed by diagnostic imaging
Implementation Method 2
formulation comprises Carbopol, triethanolamine, isopropyl alcohol, glycerin, and Nipagin
Implementation Method 3
involving histological fixation, dehydration, cutting, and staining processes
Implementation Method 4
The solution enables safe, long-term preservation and analysis of biological tissues
Implementation Method 5
formulated with Carbopol, triethanolamine, isopropyl alcohol, glycerin, and Nipagin, allowing for indefinite preservation and analysis via diagnostic imaging
Data Source
AI summary
A process for making and using a tissue preservation gel. The gel is comprised of water, reticulated acrylic acid, water soluble short-chain paraben, isopropyl alcohol and either triethanolamine or propylene glycol. To use the gel, a tissue to be preserved is first bathed in hypertonic sodium chloride, then soaked in a wash in multiple increasing concentrations of isopropyl alcohol and then preserved indefinitely in the tissue preservation gel.