Tissue Sectioning System with Merged Microscope Frame
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Solution Overview
Problem
Existing tissue sectioning systems face limitations in processing larger samples efficiently and effectively, and they often require complex protocols and large spaces, which restrict their universal coupling with existing microscope systems.
Innovation Solution
A novel tissue sectioning system is developed that includes a mounting frame extension movable along the X-Y-Z axes independently, allowing for iterative sectioning-imaging processes without obstructing the microscope's imaging region. This system uses a double-layered liquid system for optical clearing and includes a sample tank for immersion in clearing reagents, enhancing imaging quality and sample handling.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If traditional tissue sectioning systems are used, then sample processing can be performed, but the systems require large spaces and complex protocols that restrict universal coupling with microscope systems
Solution Approach 1:
The tissue sectioning system is merged with the microscope system by integrating the sectioning apparatus directly onto the microscope frame. The mounting frame attaches to the microscope's base or stage, allowing the blade to access samples positioned on the microscope stage without requiring separate processing equipment or large additional space.
Solution Approach 2:
The mounting frame is designed with universal coupling capabilities that can interface with various microscope models. The frame includes adjustable mounting mechanisms and standardized attachment points that accommodate different microscope configurations, enabling the sectioning system to be adapted to existing microscope systems without requiring custom-built solutions for each microscope type.
2Measurement precision
If samples are fixed on an x-y plane for imaging, then image capture can be performed, but the field of view is fixed and sample distortion occurs during longtime imaging
Solution Approach 1:
The system transitions from a static fixed-plane imaging approach to a dynamic iterative sectioning-imaging process. After each imaging cycle, the blade removes a thin layer of tissue, and the stage moves the sample to present a new surface to the microscope. This dynamic adjustment allows continuous high-quality imaging throughout the sample thickness without fixed field of view limitations or distortion from prolonged imaging of a single plane.
Solution Approach 2:
The system adds the z-dimension (depth) to the traditional x-y plane imaging by implementing iterative sectioning. Instead of attempting to image the entire sample thickness in a single plane, the blade progressively removes tissue layers along the z-axis, creating new imaging surfaces. This dimensional approach allows the microscope to maintain optimal focus and resolution while capturing three-dimensional sample information through multiple sequential two-dimensional images.
3Manufacturing precision
If iterative sectioning-imaging process is implemented, then high-quality three-dimensional images of larger samples can be acquired, but the system requires precise coordination of sectioning and imaging operations
Solution Approach 1:
The sectioning and imaging operations are merged into a single integrated workflow by mounting the blade apparatus directly on the microscope frame. The blade position is mechanically linked to the stage movement, and the imaging system automatically captures images at each sectioning depth. This integration eliminates the need for separate coordination between independent sectioning and imaging devices, reducing operational complexity despite the iterative process.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The system enables the acquisition of high-quality three-dimensional images of larger samples in a timely manner, reduces sample distortion and information loss, and simplifies the sectioning process, while maintaining compatibility with various microscope systems.
Implementation Method 1
immersing thick, opaque biological tissues in an optical-clearing solution
Data Source
AI summary
The disclosure provides a tissue sectioning system for direct coupling with a microscope. The tissue sectioning system includes a frame having a blade module, a container containing a solution system, and a movable arm. Specifically, the blade module is used to slice a sample. The solution system in the container includes at least two solutions, which are the first and second solutions. Further, a refractive index of the first and second solutions is identical, and the first and second solution is immiscible with each other. The first moveable arm has two ends, which are the first end and second ends. The first and second ends connect to the container and the frame, respectively. Additionally, the first moveable arm moves the container along the Z-axis.