Tissue Viability Preservation Using Biomembrane Sealing Agents
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Solution Overview
Problem
Current methods for preserving biological tissues and organs are limited by their short viability post-harvest, making it challenging to store and transport them for extended periods, leading to a mismatch between tissue availability and demand in transplantation, with most tissues remaining viable for only a few days to weeks.
Innovation Solution
Development of novel compositions and methods using biomembrane sealing agents and specific biochemical agents to create tissue-viability-preserving solutions that maintain cellular viability and tissue integrity for extended periods, allowing storage and transport of mammalian cells, tissues, and organs for several hours to several months.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Duration of action of stationary object
If conventional buffer solutions and tissue storage solutions are used, then tissues can be preserved for a short period (a few hours to a few days), but the viability and suitability of the tissue is lost after this period
Solution Approach 1:
The patent changes the chemical and physical parameters of the storage solution by incorporating cryoprotectants, antioxidants, and specific ion compositions to extend the viability period from days to months while maintaining tissue suitability for transplant
Solution Approach 2:
The invention uses composite preservation solutions combining multiple agents (cryoprotectants, antioxidants, buffers, and ions) to achieve extended viability and maintain tissue functionality throughout the storage period
2Duration of action of stationary object
If tissues are stored and transported under ideal conditions, then some viability is maintained, but the post-harvest time interval is limited to a few days to weeks
Solution Approach 1:
The patent applies preliminary protective measures immediately after harvest by treating tissues with preservation solutions containing cryoprotectants and antioxidants, which prevents degradation before the tissue can be stored or transported, thereby extending the window of opportunity
Solution Approach 2:
The invention introduces intermediary preservation solutions that act as mediators between harvest and transplant, maintaining tissue viability during the transition period and effectively extending the usable time window
3Loss of time
If the time interval from harvest to transplant is extended to several weeks or months, then more time is available for matching and transport, but conventional solutions cannot maintain cellular viability
Solution Approach 1:
The patent modifies storage solution parameters to enable long-term viability by adjusting temperature, chemical composition, and adding protective agents that prevent cellular degradation over extended periods
4Quantity of substance
If tissues are stored for extended periods, then the supply of viable explants can be increased, but conventional methods result in rapid loss of viability and function
Solution Approach 1:
The invention maintains continuous protective action through the preservation solution that continuously prevents cellular degradation and maintains viability throughout the entire storage and transport period, enabling extended availability of viable explants
Data Source
AI summary
Disclosed are methods for the preservation and storage of living biological tissues, organs, and populations of isolated cells. Also disclosed are compositions and methods to permit biological samples (including e.g., cells, cell cultures, tissues, and organs) to be harvested from suitable donor animals, stored for prolonged periods under refrigerated, cryogenic, or near-freezing, and then transported and implanted into a site within the body of a selected recipient animal, all without significant loss of cellular viability, tissue integrity, and/or biochemical function of the stored biological sample.