Biological Tissue Observation Using Vibration Optical Activity Spectroscopy
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Solution Overview
Problem
Current methods cannot effectively evaluate the quality of regenerated cartilage tissues, which are transplanted without prior assessment, due to their complex structure and difficulty in analysis using conventional spectroscopic methods.
Innovation Solution
A technique utilizing pulsed terahertz waves and vibration optical activity spectroscopy to noninvasively evaluate the dynamic physical properties of cartilage and regenerated cartilage tissues by measuring the state of water molecules and molecular vibrations, enabling the estimation of tissue formation and quality.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional spectroscopic methods are used to analyze cartilage tissue, then the analysis can be performed with standard equipment, but the complex structure of cartilage tissue makes it difficult to obtain meaningful evaluation data
Solution Approach 1:
The patent changes the wavelength parameter of the probe light to the near-infrared region (700-2500 nm), which allows deeper penetration into cartilage tissue and provides better interaction with water molecules and extracellular matrix components. This parameter change enables the detection of dynamic physical properties that are not accessible with conventional visible light spectroscopy, thereby improving measurement precision for tissue quality evaluation.
Solution Approach 2:
The patent uses water molecules trapped in the extracellular matrix as an intermediary to indirectly evaluate tissue quality. By measuring the dynamic physical properties of water molecules through near-infrared spectroscopy, the system can assess cartilage tissue health without directly analyzing the complex macromolecular structure, thus overcoming the measurement difficulty while maintaining high precision.
2Reliability
If regenerated cartilage is transplanted without noninvasive quality evaluation, then the transplantation process is simpler and faster, but the quality and safety of the transplanted tissue cannot be ensured
Solution Approach 1:
The near-infrared spectroscopy system enables the cartilage tissue itself to provide information about its quality through its intrinsic optical properties. The water molecules and extracellular matrix components in the tissue naturally interact with the probe light, generating signals that reflect tissue health status. This self-service approach eliminates the need for complex external evaluation procedures while ensuring reliable quality assessment.
Solution Approach 2:
The patent replaces complex mechanical evaluation methods (such as histological analysis or physical testing) with optical measurement using near-infrared spectroscopy. This substitution simplifies the evaluation system while maintaining or improving reliability, as the optical method is noninvasive, rapid, and provides real-time feedback on tissue quality.
3Loss of information
If terahertz waves are used to obtain information on intermolecular and intramolecular vibrations, then detailed molecular information is obtained, but the signal is hidden by noise in intensity or energy spectrum making it difficult to detect
Solution Approach 1:
The patent shifts the wavelength parameter from the terahertz region to the near-infrared region (700-2500 nm). This parameter change moves the measurement away from the noisy terahertz region while still accessing molecular vibration information, as the near-infrared region contains overtone and combination bands of fundamental molecular vibrations. This resolves the detection difficulty while preserving molecular information.
Solution Approach 2:
The patent uses water molecules as an intermediary to detect molecular vibration information indirectly. By measuring the spectral features of water in the near-infrared region, which is influenced by the surrounding macromolecular structure and dynamics, the system obtains information about intermolecular and intramolecular vibrations without directly measuring the weak terahertz signals, thus avoiding the noise problem.
Data Source
AI summary
An object is to provide a technique that can evaluate biological tissues such as cartilage tissue and regenerated tissues such as regenerated cartilage. A method for observing a dynamic physical property of a biological tissue according to the present invention is that a biological tissue is irradiated with a pulsed light having a wavelength of a far-infrared wavelength region modulated into circular polarized lights by applying bias voltages to a radiation means (3) having an antenna electrode films of orthogonal (2)-axis structure with phases shifted using high-voltage high-speed modulation means (13), and dynamic physical property of the biological tissue is observed on the basis of a spectrum obtained by vibration optical activity spectroscopy.