TNAP Marker Isolation of Adult Multipotential Cells
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Solution Overview
Problem
Current methods lack effective markers for isolating and enriching adult multipotential cells, particularly bone marrow mesenchymal precursor cells, which are crucial for tissue regeneration and therapy, due to the absence of well-defined markers and limited monoclonal antibodies.
Innovation Solution
Utilizing tissue non-specific alkaline phosphatase (TNAP) as a marker, identified by the STRO-3 monoclonal antibody, to selectively enrich adult multipotential cells through binding agents like antibodies or collagen, allowing for their isolation and enrichment from various tissue sources.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If traditional markers like CBFA1 are used to identify multipotential cells, then osteogenic commitment can be detected, but the cells cannot be isolated and manipulated within heterogeneous populations
Solution Approach 1:
The patent introduces an intermediary approach by using a combination of markers (STRO-1, CD105, CD73, CD90) rather than relying on a single marker. This intermediary system allows for both detection and isolation capabilities, bridging the gap between precise identification and practical cell manipulation in heterogeneous populations.
2Measurement precision
If STRO-1 monoclonal antibody is used to identify all multipotential cells, then comprehensive identification is achieved, but the antibody is down regulated following ex vivo culture and loses effectiveness
Solution Approach 1:
The patent applies parameter changes by shifting from reliance on a single marker (STRO-1) to a multi-marker profile (STRO-1, CD105, CD73, CD90). This parameter diversification allows the system to maintain identification precision even when one marker's expression changes during ex vivo culture, thereby extending the effective duration of cell identification.
3Measurement precision
If multiple markers are used to identify multipotential cells, then identification accuracy improves, but the complexity of isolation methods increases
Solution Approach 1:
The patent merges multiple identification markers (STRO-1, CD105, CD73, CD90) into a unified cell isolation protocol. By combining these markers into a single multi-parameter identification system, the method achieves high identification accuracy while maintaining practical simplicity in the isolation process, avoiding the need for separate complex procedures for each marker.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The STRO-3 antibody effectively isolates and enriches adult multipotential cells, maintaining their undifferentiated state and enhancing their expansion capacity, facilitating their use in therapeutic applications and tissue regeneration.
Implementation Method 1
The STRO-3 antibody effectively isolates and enriches adult multipotential cells
Data Source
AI summary
The present invention relates to the use of tissue non-specific alkaline phosphatase (TNAP) as a marker for identifying and/or isolating adult multipotential cells. The present invention also relates to cell populations enriched by methods of the present invention and therapeutic uses of these cells.