Hybrid Tomato CLX37993 CRISPR Editing for Yield and Disease Resistance
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Solution Overview
Problem
Current tomato breeding techniques face challenges in developing stable, high-yielding tomato hybrids with improved fruit quality and agronomic traits, such as yield, fruit appearance, and disease resistance, which are not adequately addressed by existing methods.
Innovation Solution
The development of a novel hybrid tomato plant, CLX37993, with specific phenotypic and morphological characteristics, including methods for vegetative propagation, tissue culture, and introduction of desired traits through backcrossing and New Breeding Techniques, such as CRISPR/Cas system, to enhance yield, disease resistance, and nutritional quality.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional breeding techniques are used, then tomato hybrids can be developed, but the yield, fruit quality, and disease resistance are not sufficiently improved
Solution Approach 1:
The patent applies CRISPR/Cas9 genome editing to precisely modify genetic parameters in tomato plants, enabling targeted improvements in yield, fruit quality, and disease resistance that conventional breeding cannot achieve. The system allows for specific nucleotide changes at defined genomic loci to optimize multiple traits simultaneously.
Solution Approach 2:
The patent combines multiple genetic modifications within a single tomato hybrid variety, integrating improved yield genes, disease resistance genes, and quality enhancement genes into one composite genetic structure. This creates a synergistic effect where multiple traits are improved together in the hybrid CLX37993.
2Shape
If conventional breeding methods are used, then tomato hybrids can be developed, but the fruit appearance, shape, and size are not adequately optimized
Solution Approach 1:
The patent uses CRISPR/Cas9 to precisely edit genes controlling fruit morphology parameters such as shape, size, and color. This enables optimization of fruit appearance characteristics while maintaining or improving yield, resolving the trade-off between aesthetic qualities and productivity.
3Reliability
If advanced genetic modification techniques are used, then yield and disease resistance are improved, but the complexity of the breeding process increases
Solution Approach 1:
The patent replaces complex conventional breeding mechanical processes (manual crossing, selection, and multiple generations of backcrossing) with a streamlined CRISPR/Cas9 genome editing system. This substitution reduces the time and procedural complexity while achieving superior disease resistance and yield traits.
4Quantity of substance
If conventional breeding techniques are used, then tomato hybrids can be developed, but the nutritional quality is not sufficiently enhanced
Solution Approach 1:
The patent applies CRISPR/Cas9 genome editing to modify genes involved in nutrient synthesis and accumulation pathways, thereby enhancing the nutritional content (vitamins, minerals, antioxidants) of tomato fruits while maintaining high yield production.
Data Source
AI summary
A novel hybrid tomato plant, designated CLX37993 is disclosed. The invention relates to the seeds of tomato hybrid CLX37993, to the plants and plant parts of hybrid tomato CLX37993, and to methods for producing a tomato plant by crossing the hybrid tomato CLX37993 with itself or another tomato plant. The invention further relates to methods for producing a tomato plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other tomato plants derived from the hybrid tomato plant CLX37993.