Tomato Pathogen Detection Using Artificial Cell Wall
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Solution Overview
Problem
Current methods for detecting tomato pathogenic fungi often result in false positives due to the detection of non-specific phytopathogenic fungi, leading to unnecessary chemical applications and seedling renewal in tomato cultivation.
Innovation Solution
A tomato pathogenic fungus detecting apparatus featuring an artificial cell wall with a 50 mM to 70 mM citrate salt buffer solution at pH 5 to 5.5, which selectively allows tomato pathogenic fungi to penetrate while excluding non-pathogenic fungi by controlling the hole diameter and membrane thickness of the cellulose membrane.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If a pathogenic fungus selection technique using an artificial cell wall is used to detect tomato pathogenic fungi, then detection capability is improved, but false positives occur due to detection of non-specific phytopathogenic fungi
Solution Approach 1:
The invention applies local quality by creating a detection environment with specific local conditions (pH 5-5.5 buffer solution, specific nutrient composition) that are optimized for tomato pathogenic fungi. The artificial cell wall is positioned in a specific location within the detection chamber, and the pH buffer is applied locally to the test sample solution, creating a selective microenvironment that distinguishes target fungi from non-target phytopathogenic fungi.
Solution Approach 2:
The invention changes key parameters of the detection environment, specifically setting the pH to 5-5.5 using a citrate buffer solution and controlling nutrient composition in the culture medium. These parameter changes create conditions that favor the growth and penetration of tomato pathogenic fungi while inhibiting or slowing down non-specific phytopathogenic fungi, thereby reducing false positives while maintaining detection capability.
2Measurement precision
If the artificial cell wall membrane thickness is reduced to allow fungal penetration, then detection sensitivity is improved, but selectivity decreases allowing non-pathogenic fungi to penetrate
Solution Approach 1:
The invention introduces an intermediary system consisting of the pH buffer solution and specific nutrient composition that mediates between the artificial cell wall membrane and the fungal hyphae. This intermediary environment creates selective pressure that allows tomato pathogenic fungi to penetrate the membrane while preventing or slowing penetration by non-pathogenic fungi, thus maintaining both sensitivity and selectivity.
Solution Approach 2:
The invention uses a composite detection system combining the artificial cell wall membrane with a specially formulated pH buffer solution and culture medium. This composite approach integrates multiple functional components: the membrane provides physical filtration, the pH buffer provides chemical selection, and the nutrients provide biological selection, together achieving both sensitivity and selectivity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables safe and simple selective detection of tomato pathogenic fungi before pathogenesis, preventing false positives and reducing unnecessary agricultural interventions.
Implementation Method 1
a test sample solution contains a 50 mM to 70 mM buffer solution of a citrate salt in the test sample solution inlet, and the test sample solution has a pH of 5 to 5.5
Data Source
AI summary
The present disclosure provides a simple and secure apparatus and a simple and secure method for selectively detecting a tomato pathogenic fungus. The tomato pathogenic fungus detecting apparatus according to the present disclosure is characterized by including an artificial cell wall, a test sample solution inlet provided above the artificial cell wall, and a culture solution storage part provided under the artificial cell wall, wherein a test sample solution contains a 50 mM to 70 mM buffer solution of a citrate salt in the test sample solution inlet, and the test sample solution has a pH of 5 to 5.5.