Trace Meter for Calibrating Ultra-Micro Pipetting Precision
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Conventional measurement and calibration devices cannot accurately measure and calibrate the ultra-micro dynamic pipetting precision of high-throughput nucleic acid extraction devices, leading to excessive differences among channels and affecting measurement results.
Innovation Solution
A trace meter with a measuring compartment, temperature sensor, pressure sensor, and controller is used to calibrate dynamic ultra-micro pipetting devices. The trace meter measures temperature and weight of liquid in the measuring compartment, allowing for precise calculation of pipetting consistency and overall pipetting precision.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional measurement and calibration devices are used, then device complexity is reduced, but measurement precision of ultra-micro dynamic pipetting deteriorates
Solution Approach 1:
The measurement system is segmented into specialized functional modules: a measuring compartment for containing the liquid sample, a temperature sensor for thermal measurement, a pressure sensor for weight measurement, and a controller for data processing. This segmentation allows each component to be optimized for its specific function, achieving ultra-micro measurement precision without requiring an overly complex integrated device.
Solution Approach 2:
The measurement device integrates multiple functions into a single system: volume measurement through weight measurement (pressure sensor), temperature measurement (temperature sensor), and automated calculation of pipetting precision (controller). This multi-functionality achieves high measurement precision while avoiding the need for multiple separate devices, thus not increasing overall device complexity.
2Productivity
If high-throughput dynamic pipetting is performed, then productivity is improved, but pipetting precision deteriorates due to excessive differences among channels
Solution Approach 1:
The controller receives measurement data from all pipetting channels, automatically calculates the coefficient of variation (CV value) for each channel, and provides feedback on pipetting precision. This feedback mechanism enables identification and calibration of channels with excessive differences, thereby maintaining high pipetting precision across all channels even during high-throughput operations.
Solution Approach 2:
The system performs self-calibration by automatically measuring each channel's pipetting precision using the trace meter, calculating CV values, and identifying channels that require adjustment. This self-service capability allows the system to maintain precision across multiple channels without requiring manual calibration of each individual channel, thus supporting high-throughput operations.
3Productivity
If long-term use of pipetting heads occurs, then productivity is maintained, but pipetting precision deteriorates due to human operation and environmental factors
Solution Approach 1:
The system implements periodic calibration by automatically measuring pipetting precision at regular intervals during continuous operation. The controller periodically collects data from all channels, calculates CV values, and triggers calibration when precision degradation is detected. This periodic action maintains pipetting precision consistency over long-term use without interrupting overall productivity.
Solution Approach 2:
The system continuously monitors pipetting precision through automated measurements and provides real-time feedback on the state of each pipetting head. When environmental factors or prolonged use cause precision degradation, the feedback mechanism identifies the affected channels and triggers recalibration, thereby maintaining consistent precision throughout long-term operation.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The trace meter improves the metering precision of ultra-micro pipetting by reducing evaporation loss and directly outputting high-precision volume values, facilitating the calibration of dynamic ultra-micro pipetting precision and enhancing the overall pipetting precision of high-throughput devices.
Implementation Method 1
The temperature sensor is configured to measure a temperature of liquid in the measuring compartment
Implementation Method 2
The pressure sensor is configured to measure a weight of the liquid in the measuring compartment
Implementation Method 3
One side, adjacent to an inside of the measuring compartment, of the measuring hole is covered with a flexible heat-conducting film
Data Source
AI summary
A trace meter and a method for calibrating a dynamic ultra-micro pipetting device are provided, which relate to the technical field of dynamic pipetting precision calibration. The trace meter includes a measuring compartment, a temperature sensor, a pressure sensor and a controller. A top of the measuring compartment is open, the temperature sensor is provided at a bottom of the measuring compartment, the pressure sensor is provided below the measuring compartment, both the temperature sensor and the pressure sensor are electrically connected with the controller, the temperature sensor is configured to measure the temperature of liquid in the measuring compartment, and the pressure sensor is configured to measure the weight of liquid in the measuring compartment.