Transformable Tagging Moieties for Nucleic Acid Barcoding
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Solution Overview
Problem
Current barcoding strategies in life sciences require large diverse libraries of oligonucleotide barcodes, which can be challenging and costly to prepare and allocate across multiple samples.
Innovation Solution
The use of transformable tagging moieties that can be transformed in situ into a collection of unique tagging or barcode moieties, eliminating the need for complex, highly diverse libraries.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If large diverse libraries of oligonucleotide barcodes are used for tagging molecular species, then the ability to differentiate and identify multiple samples is improved, but the complexity and cost of preparing and allocating barcodes increases
Solution Approach 1:
A single universal tagging moiety structure is designed that can serve multiple functions through in situ transformation. The tagging moiety includes transformable nucleotides that can be converted into diverse barcode sequences, allowing one universal structure to generate multiple specific barcodes for differentiating various molecular species without requiring pre-prepared diverse barcode libraries
Solution Approach 2:
The nucleotide composition of the tagging moiety is changed in situ through transformation processes. By modifying the nucleotide sequences after initial tagging, a single tagging event can produce multiple different barcode variants, enabling differentiation of molecular species without requiring the preparation and allocation of large diverse barcode libraries beforehand
2Adaptability or versatility
If large diverse libraries of oligonucleotide barcodes are used for tagging molecular species, then the ability to differentiate and identify multiple samples is improved, but the cost of preparing and allocating barcodes increases
Solution Approach 1:
Instead of physically preparing and allocating many different barcode molecules, the invention uses a single tagging moiety that copies itself with variations through in situ transformation. The transformable nucleotides allow the same tagging structure to generate multiple barcode copies with different sequences, reducing the need to synthesize and allocate large quantities of diverse barcode substances
Solution Approach 2:
The chemical composition parameters of the tagging moiety, specifically the nucleotide sequences, are changed after the initial tagging event. This post-tagging transformation allows one tagging event to produce multiple barcode variants, eliminating the need to prepare and allocate large quantities of diverse barcode molecules, thereby reducing material costs
3Device complexity
If transformable tagging moieties are used to generate diverse barcodes in situ, then the complexity and cost of barcode preparation is reduced, but the transformation process must be controlled to ensure unique tagging
Solution Approach 1:
The tagging system incorporates dynamic transformation of nucleotides after initial tagging. The transformable nucleotides can be converted into different bases through controlled processes, allowing the system to adapt and ensure uniqueness of each barcode. This dynamic adjustment capability enables precise control over the transformation process to achieve accurate differential tagging without requiring complex pre-prepared libraries
Data Source
AI summary
Provided herein are compositions, systems and methods for tagging molecular events, reactions, species, etc., but without the need for complex, highly diverse libraries of tagging molecules. Provided are tagging moieties that can have a smaller number, a few, or even a single original “tagging” structure that may be transformed or transformable, in situ, into a collection of larger numbers of unique tagging or “barcode” moieties.