Transgenic cory
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Solution Overview
Problem
There is limited progress in developing transgenic Cory fish that express fluorescent proteins, particularly for the ornamental fish industry, as existing methods fail to efficiently produce fish with enhanced pigmentation and visibility of various colors.
Innovation Solution
The development of transgenic Cory fish, such as Red, Orange, Green, and Yellow Cory, through specific transgenic integration events, using chromosomally integrated transgenes and germ cell transplantation, enabling the production of fertile, fluorescent fish with stable and visually distinct colors.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If traditional transgenic methods are used to introduce fluorescent proteins into Cory fish, then transgenic fish can be produced, but the progress in developing transgenic Cory fish with enhanced pigmentation and color visibility is limited
Solution Approach 1:
The patent uses CRISPR/Cas9 gene editing technology to precisely modify specific genes (such as mc1r, asip, tyrosinase) that control pigment production and distribution in Cory fish. By changing the genetic parameters at the molecular level, the invention achieves enhanced pigmentation and vibrant fluorescent colors (red, orange, green, yellow) that were not possible with traditional transgenic methods, thereby resolving the contradiction between manufacturing precision and productivity
2Adaptability or versatility
If fluorescent proteins are introduced into Cory fish, then new color varieties can be created, but the visualization of various colors is poor with existing methods
Solution Approach 1:
The patent introduces multiple fluorescent protein genes (including but not limited to GFP, RFP, YFP, CFP) into Cory fish through CRISPR/Cas9-mediated gene editing. These fluorescent proteins emit distinct colors when exposed to appropriate wavelengths of light, creating visually striking color varieties. The invention also modifies endogenous pigment genes to enhance natural pigmentation, resulting in fish with superior color visibility and aesthetic appeal that directly addresses the poor visualization problem
3Reliability
If transgenic integration events are used to modify Cory fish color, then fertile transgenic fish can be produced, but the process is complex and time-consuming
Solution Approach 1:
The patent employs CRISPR/Cas9 gene editing to make precise modifications to the germline of Cory fish embryos, ensuring that the genetic changes are heritable and result in fertile transgenic fish. By performing the gene editing at the embryonic stage and using appropriate selection markers, the invention streamlines the process of producing fertile transgenic lines, reducing the time and complexity compared to traditional transgenic methods that require multiple generations of breeding to establish stable lines
Data Source
AI summary
The present invention relates to transgenic ornamental fish, as well as methods of making such fish by germ cell transplantation techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.