Transgenic Maize Gene Stacking for Pest and Herbicide Resistance
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Solution Overview
Problem
Current methods for controlling corn borers and weeds in maize fields rely heavily on pesticides and herbicides, which increase costs and harm the environment, while transgenic maize offers resistance but requires specific gene integration and detection methods to enhance yield and quality.
Innovation Solution
Development of a nucleic acid molecule comprising specific sequences for expressing glufosinate-resistant, insect-resistant, and glyphosate-resistant genes in maize, along with probes and primer pairs for detecting transformation events, enabling the creation of transgenic maize lines resistant to pests and herbicides.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Object-affected harmful factors
If pesticides and herbicides are used to control corn borers and weeds, then pest and weed control is achieved, but planting costs increase and environmental harm occurs
Solution Approach 1:
The maize plant is genetically modified to produce its own insect-resistant proteins (Cry1Ab, Cry1Ac, Cry2A2) and herbicide resistance (glufosinate, glyphosate), enabling it to defend itself against pests and weeds without external chemical inputs, thereby reducing planting costs and environmental harm
Solution Approach 2:
The resistance genes are integrated into the maize genome in advance through transformation, so the plant possesses inherent resistance before pest or weed infestation occurs, eliminating the need for reactive pesticide and herbicide applications
2Productivity
If transgenic maize with insect-resistant genes is developed, then yield and quality are improved, but specific gene integration and detection methods are required
Solution Approach 1:
The patent integrates multiple resistance functions into a single transgenic system, combining insect resistance (Cry1Ab, Cry1Ac, Cry2A2 genes) and herbicide resistance (glufosinate, glyphosate genes) in one maize variety, allowing simultaneous protection against multiple pests and weeds while simplifying breeding and deployment
Solution Approach 2:
The patent provides specific detection primers and probes (SEQ ID NOs: 2-11) that act as intermediaries to identify and verify the presence of transgenic resistance genes in maize plants, simplifying the detection process and enabling routine screening of transgenic events
Data Source
AI summary
An insect-resistant herbicide-tolerant corn transformation event, and a related creation method, a detection method, and application thereof are provided herein. Using the corn inbred line Xiang 249 as a receptor, by means of agrobacterium-mediated genetic transformation, obtaining a corn plant with an exogenous gene insert inserted at a specific genomic locus, the exogenous gene insert comprising the following three genes: an insect-resistant gene, a glufosinate resistant gene, and a glyphosate resistant gene. In the obtained transformation event, the inserted exogenous genes are positioned at a non-functional locus of the corn genome, and do not affect the expression of the other genes of the receptor plant, such that the transgenic corn plant maintains good agronomic traits whilst acquiring insect resistance and herbicide tolerance.