Transgenic Plants With Recombinant DNA Constructs for Yield Under Stress
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Solution Overview
Problem
Existing agricultural practices face challenges in enhancing crop yield, nitrogen use efficiency, and water use efficiency in plants, particularly under stress conditions such as drought and nitrogen limitation.
Innovation Solution
Development of recombinant DNA constructs comprising specific polynucleotide sequences with high identity to SEQ ID NOs: 1-39, which are integrated into plant genomes using site-directed integration, modulating endogenous gene expression to alter phenotypes and enhance traits, thereby increasing yield, nitrogen use efficiency, and water use efficiency.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional breeding and traditional agricultural practices are used, then crop yield and efficiency can be improved to some extent, but the improvement is limited and cannot meet the demands of growing population and resource constraints
Solution Approach 1:
The patent changes the genetic parameters of plants by introducing recombinant DNA constructs containing specific polynucleotide sequences (SEQ ID NOs: 1-39) that encode proteins involved in stress response and metabolic pathways. This modifies the plant's genetic makeup to enhance productivity and stress tolerance beyond what conventional breeding can achieve.
Solution Approach 2:
The patent creates composite genetic structures by combining multiple polynucleotide sequences into recombinant DNA constructs. These constructs integrate multiple functional elements (promoters, coding sequences, regulatory regions) to simultaneously improve multiple traits including yield, nitrogen use efficiency, and stress tolerance.
2Productivity
If conventional breeding methods are used, then some trait improvement is achieved, but the process is time-consuming and cannot rapidly introduce multiple desirable traits
Solution Approach 1:
The patent performs preliminary action by pre-assembling recombinant DNA constructs containing multiple desirable traits before introduction into plants. This allows simultaneous transfer of multiple traits (yield improvement, stress tolerance, nutrient use efficiency) in a single transformation event, bypassing the sequential selection process of conventional breeding.
Solution Approach 2:
The patent replaces the mechanical breeding process (crossing, selection, and repeated backcrossing) with a molecular biology approach using recombinant DNA technology. This substitution enables direct genetic modification to achieve trait improvement without the time-consuming mechanical steps of traditional breeding.
3Reliability
If traditional agricultural practices are used, then crops can grow under normal conditions, but they show reduced performance under stress conditions such as drought and nitrogen limitation
Solution Approach 1:
The patent applies local quality by introducing specific polynucleotide sequences that target particular stress response pathways and metabolic processes. The recombinant DNA constructs are designed to express proteins in specific tissues or at specific developmental stages to enhance stress tolerance without compromising overall plant productivity.
Solution Approach 2:
The patent converts the harmful effect of stress conditions (drought, nitrogen limitation) into beneficial outcomes by introducing genes that enable plants to utilize stress conditions more effectively. The recombinant DNA constructs allow plants to maintain or even improve productivity under stress by activating alternative metabolic pathways and stress protection mechanisms.
Data Source
AI summary
This disclosure provides recombinant DNA constructs and transgenic plants having enhanced traits such as increased yield, increased nitrogen use efficiency, and enhanced drought tolerance or water use efficiency. Transgenic plants may include field crops as well as plant propagules, plant parts and progeny of such transgenic plants. Methods of making and using such transgenic plants are also provided. This disclosure also provides methods of producing seed from such transgenic plants, growing such seed, and selecting progeny plants with enhanced traits. Also disclosed are transgenic plants with altered phenotypes which are useful for screening and selecting transgenic events for the desired enhanced trait.