Transparent ISFET Sensor for Real-Time Cell Observation
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Solution Overview
Problem
Current biosensors face challenges in optically observing cell behavior due to their opaque nature, limiting the real-time detection of ion concentration variations and metabolic activities of cells.
Innovation Solution
A cell-based transparent sensor is developed, comprising a transparent substrate with an ion-selective field effect transistor (ISFET) sensor and an oxygen detection electrochemical sensor, using transparent electrodes, semiconductor layers, and ion-sensitive membranes, allowing for real-time optical observation of cell behavior and ion concentration measurements.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Illumination intensity
If conventional opaque biosensors are used, then electrical signal detection is achieved, but optical observation of cell behavior is blocked
Solution Approach 1:
The patent changes the optical parameter (transparency) of the sensor components by selecting transparent materials for electrodes, semiconductor layers, and insulator layers, while maintaining their electrical and sensing functions through careful material selection and layer design
Solution Approach 2:
The patent employs composite material structures where transparent conducting oxides, transparent semiconductor materials, and transparent ion-sensitive insulators are combined to create a sensor that simultaneously achieves optical transparency and electrical functionality for reliable detection
2Illumination intensity
If transparent materials are used for sensor components, then optical observation is enabled, but manufacturing complexity increases
Solution Approach 1:
The patent divides the sensor into distinct functional layers (transparent electrode layer, transparent semiconductor layer, transparent ion-sensitive insulator layer, ion-selective membrane) that can be manufactured separately using standard semiconductor fabrication techniques and then integrated, simplifying the overall manufacturing process
Solution Approach 2:
The patent designs transparent sensor components that serve multiple functions - the transparent ion-sensitive insulator layer provides both optical transparency and ion sensing functionality, while transparent electrodes provide both electrical conduction and optical transparency, reducing the need for additional components
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables accurate, real-time optical observation and electrochemical detection of cell behavior, facilitating the monitoring of ion concentrations and metabolic changes, with applications in performance evaluation of medicines, environmental monitoring, and clinical diagnosis.
Implementation Method 1
an ion-selective field effect transistor (ISFET) sensor disposed on the transparent substrate... detect an ionic concentration of an electrolyte that corresponds to a variation in the metabolic activity of cells
Implementation Method 2
an oxygen detection electrochemical sensor disposed on the transparent substrate in parallel to the ion-selective field effect transistor sensor
Data Source
AI summary
The present invention relates to a cell-based transparent sensor capable of the real-time optical observation of cell behavior, to a method for manufacturing same, and to a multi-detection sensor chip using same. More particularly, the present invention relates to a cell-based transparent sensor capable of the real-time optical observation of cell behavior, to a method for manufacturing same, and to a multi-detection sensor chip using same, wherein the sensor can sense the ionic concentration of an electrolyte in accordance with the variation in the metabolic activity of cells using an ion-selective field effect transistor (ISFET) sensor and an electrochemical sensor, and the sensor is made of a transparent material which enables real-time observations of optical phenomenon for measurement of cell behavior.