Trap Column Dilution with Additive Agent to Prevent Precipitation
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Solution Overview
Problem
Conventional preparative separation/purification systems face challenges in efficiently capturing target compounds without causing clogging in the trap column or pipes, due to the decreased solubility and increased precipitation of both target and foreign compounds when the mobile phase is diluted, leading to inefficiencies and losses in capturing pharmacological compounds.
Innovation Solution
A preparative separation/purification system that includes a trap column with a diluting liquid supply passage for mixing a diluting liquid with a sample-introducing solvent, and an additive-mixing means to incorporate an additive agent that prevents precipitation, allowing for controlled adjustment of the elution capability and solubility to ensure efficient capture of target compounds without clogging.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If the mobile phase is diluted with water to lower its elution capability, then the target compound is more strongly held on the adsorbent, but the solubility of both target and foreign compounds decreases causing precipitation and clogging
Solution Approach 1:
The patent introduces a third substance (additive agent) that acts as an intermediary between the diluting liquid and the compounds in the mobile phase. This additive agent prevents precipitation of both target and foreign compounds while allowing the diluting liquid to reduce the elution capability of the mobile phase, thus enabling effective capturing without clogging
Solution Approach 2:
The patent changes the chemical composition parameters of the mobile phase by adding specific additives (such as formic acid, acetic acid, or triethylamine) in controlled amounts. This parameter change allows the system to maintain compound solubility while still achieving reduced elution capability through water dilution, preventing precipitation and clogging
2Reliability
If the mobile phase is diluted with water to lower its elution capability, then the target compound is more strongly held on the adsorbent, but foreign compounds also precipitate causing clogging in pipes and trap column
Solution Approach 1:
The additive agent serves as a protective intermediary that prevents foreign compounds from precipitating and being lost in the clogged material. It maintains their solubility in the diluted mobile phase, allowing them to be properly separated and collected rather than lost as precipitate
3Reliability
If trapping conditions are optimized for each compound, then the capturing efficiency is improved, but the time, labor and cost required to determine optimal conditions increases significantly
Solution Approach 1:
The patent creates a universal trapping system where a single trap column configuration with standardized additive agent addition can effectively capture a wide variety of different compounds. The additive agents (formic acid, acetic acid, triethylamine) work across multiple compound types, eliminating the need for extensive optimization for each individual compound while maintaining high capturing efficiency
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The system effectively captures target compounds with high efficiency, preventing clogging and ensuring stable operation for a variety of compounds, allowing for efficient collection in solid forms.
Implementation Method 1
a target component contained in a liquid sample is captured by a trap column
Implementation Method 2
an eluting solvent is subsequently supplied into the trap column to elute the target component
Implementation Method 3
the solvent in an eluate is vaporized to obtain the target component in solid forms
Data Source
AI summary
A mixed liquid passage (31) is connected to a trapping passage (22) at a point upstream of a trap column (23). A mixture of a diluting liquid and an additive agent, which are respectively drawn and sent by pumps (35) and (36), is supplied into the mixed liquid passage (31). When an eluate containing a target compound is passed through the trap column (23), the eluate is diluted with the diluting liquid and hence its elution capability decreases, so that the target compound can be easily captured in the trap column (23). Meanwhile, the solubility of the mobile phase in the eluate decreases, causing not only the target component but also foreign compounds to more easily precipitate. However, their precipitation is prevented by the effect of the additive agent. Thus, clogging of the trap column (23) or pipes is prevented.