Trichoderma harzianum ThLml Inoculation for Crop Stress Tolerance

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Solution Overview

Problem

Current agricultural practices face challenges in maintaining high crop yields due to abiotic stresses like drought, temperature extremes, and salinity, with no commercially available stress-tolerant crop plants or products capable of generating such crops through breeding or genetic modification.

Innovation Solution

Inoculation of plants with the isolated Trichoderma harzianum strain ThLml, deposited under NRRL 50846, or its asexual progeny/spores, which promotes stress tolerance, enhances growth, and increases germination, thereby improving plant health and yield under stressful conditions.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If conventional agricultural practices are used, then crop production continues, but crop yields decrease due to abiotic stresses like drought, temperature extremes, and salinity

Engineering Contradiction:
Improvecrop yieldVSAvoidabiotic stress
Core Design Contradiction:
ProductivityVSObject-affected harmful factors

Solution Approach 1:

The patent applies preliminary action by inoculating plant seeds with Trichoderma harzianum ThLml fungus before planting, allowing the fungus to establish a protective symbiotic relationship with the plant before abiotic stresses occur. This pre-colonization enables the plant to have enhanced stress tolerance mechanisms already in place when drought, temperature extremes, or salinity conditions arise, thereby maintaining crop yields under stressful conditions

Inventive Principle:
Principle #10Preliminary action

2Reliability

If breeding or genetic modification approaches are used, then stress-tolerant crops could be developed, but no commercially available products currently exist capable of generating such crops

Engineering Contradiction:
Improvestress toleranceVSAvoidcommercial availability
Core Design Contradiction:
ReliabilityVSEase of manufacture

Solution Approach 1:

The patent uses Trichoderma harzianum ThLml fungus as an intermediary organism that mediates stress tolerance in plants without requiring direct genetic modification of the crop plants themselves. The fungus acts as a biological mediator that colonizes plant roots and provides protective effects against abiotic stresses, offering a commercially viable alternative to breeding or GM approaches while achieving reliable stress tolerance

Inventive Principle:
Principle #24Intermediary (Mediator)

3Productivity

If water consumption is reduced through inoculation, then plant growth is enhanced, but resource allocation requires optimization

Engineering Contradiction:
Improveplant growthVSAvoidwater consumption
Core Design Contradiction:
ProductivityVSQuantity of substance

Solution Approach 1:

The patent applies parameter changes by altering the plant's physiological parameters through fungal inoculation. The Trichoderma harzianum ThLml fungus changes the plant's water use efficiency parameter, enabling the plant to maintain enhanced growth and photosynthetic activity while consuming less water. This parameter transformation allows the plant to achieve better productivity with reduced resource input

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentEP3024925B1Compositions and method related to isolated endophytes
Publication Date: 2020.03.25 ADAPTIVE SYMBIOTIC TECHNOLOGIES LLC
  • EP3024925B1 patent drawingFigure 1~2
  • EP3024925B1 patent drawingFigure 3~4
  • EP3024925B1 patent drawingFigure 5

AI summary

Described herein are compositions and methods related to isolated Trichoderma harzianum and strains thereof.