Trichoderma Strains for Nitrogen Use Efficiency and Plant Growth

Resolve Bottlenecks,
Find Innovative Solutions
Generate Solutions

Solution Overview

Problem

Current Trichoderma strains used for plant growth promotion and disease resistance are not universally effective, and their application often results in overuse of nitrogen fertilizers, leading to environmental issues like nitrous oxide emissions and excess nitrogen in soil and water.

Innovation Solution

The use of specific Trichoderma harzianum strain RR17Bc, Trichoderma atroviride strain WW10TC4, and Trichoderma harzianum strain F11Bab, which colonize plant roots to enhance growth, resistance to abiotic stresses, and nitrogen use efficiency, thereby reducing nitrous oxide emissions and nitrogen leaching.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If current Trichoderma strains are used for plant growth promotion, then plant growth is enhanced, but nitrogen fertilizer overuse occurs leading to environmental pollution

Engineering Contradiction:
Improveplant growthVSAvoidnitrous oxide emissions and nitrogen leaching
Core Design Contradiction:
ProductivityVSObject-generated harmful factors

Solution Approach 1:

The patent applies parameter changes by selecting specific Trichoderma strains (T. harzianum RR17Bc, T. atroviride WW10TC4, T. harzianum F11Bab) with optimized characteristics for nitrogen use efficiency. These strains have been characterized to possess specific biological parameters that enhance plant nitrogen uptake and utilization, thereby reducing the need for excessive nitrogen fertilizer application and subsequent environmental pollution.

Inventive Principle:
Principle #35Parameter changes

2Quantity of substance

If Trichoderma strains are applied to increase nitrogen use efficiency, then nitrogen fertilizer requirements are reduced, but plant growth promotion effectiveness varies

Engineering Contradiction:
Improvenitrogen fertilizer requirementsVSAvoidplant growth promotion effectiveness
Core Design Contradiction:
Quantity of substanceVSReliability

Solution Approach 1:

The patent applies universality by selecting Trichoderma strains that perform multiple functions simultaneously: they promote plant growth, enhance nitrogen use efficiency, and provide disease resistance. These strains have been demonstrated to exhibit consistent effectiveness across different plant species and growth conditions, making them universally applicable solutions for both reducing nitrogen fertilizer requirements and ensuring reliable plant growth promotion.

Inventive Principle:
Principle #6Universality (Multi-functionality)

Data Source

PatentEP2393365B1Trichoderma strains that induce resistance to plant diseases and/or increase plant growth
Publication Date: 2015.05.13 CORNELL UNIVERSITY
  • EP2393365B1 patent drawingFigure 1
  • EP2393365B1 patent drawingFigure 2
  • EP2393365B1 patent drawingFigure 3

AI summary

A method for enhancing growth of plants comprising contacting a Trichoderma strain with the plant or a plant seed under conditions effective for the Trichoderma strain to colonize the roots of the plant or a plant grown from the plant seed, thereby creating a plant- Trichoderma system. The plant or plant seed is grown under conditions effective to sustain the plant-Tnchoderma system in a planting medium and to enhance plant growth. The Trichoderma strain is selected from the group consisting of Trichoderma atroviride strain WW10TC4 (ATCC accession number PTA 9707), Trichoderma harzianum strain RR17Bc (ATCC accession number PTA 9708), Trichoderma harzianum strain F11 Bab (ATCC accession number PTA 9709), and combinations thereof. Methods of enhancing resistance of plants to abiotic stress, increasing nitrogen use efficacy in plants, reducing nitrous oxide emissions in air, reducing leaching of nitrates into soil and water, and enhancing sequestration of carbon from air are disclosed.