Trichoderma reesei Mutant Strain for Enhanced Cellulase Production
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Solution Overview
Problem
Existing Trichoderma reesei strains have limitations in protein production capabilities, particularly cellulase production, and there is a need for further enhancement of protein-producing abilities through novel mechanisms.
Innovation Solution
Development of a mutant strain of Trichoderma reesei with mutations in specific polypeptides, such as those represented by SEQ ID NOs: 4, 5, and 6, to eliminate or reduce their functions, specifically targeting the HSF-type DNA-binding, TLD, and F-box domains, enhancing protein production and cellulase activity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional Trichoderma reesei strains are used for protein production, then basic cellulase production is achieved, but protein production capability is limited and cannot be further enhanced
Solution Approach 1:
The patent applies parameter changes by mutating specific genes (cre1, xyr1, atf2) that control protein production in Trichoderma reesei. By altering the genetic parameters of these transcription factors, the invention achieves enhanced cellulase and protein production capabilities beyond conventional strains, directly resolving the limitation in productivity while exploring novel genetic mechanisms.
2Productivity
If Cre1 function is reduced to enhance cellulase production, then cellulase production amount increases, but other protein production capabilities may be affected
Solution Approach 1:
The patent applies segmentation by separately optimizing multiple transcription factor genes (cre1, xyr1, atf2) rather than relying on a single gene modification. This segmented approach allows independent control over different aspects of protein production, enhancing cellulase production through Cre1 reduction while maintaining overall protein production stability through coordinated modification of multiple genetic elements.
Solution Approach 2:
The patent simultaneously modifies multiple genetic parameters (cre1, xyr1, atf2 genes) to achieve balanced protein production. By changing the functional parameters of several transcription factors in combination, the invention resolves the contradiction between enhancing cellulase production and maintaining overall protein production stability, as each gene modification contributes to different aspects of the production system.
3Productivity
If glucose or lactose is added to culture medium to improve cellulase production, then production amount increases, but production cost and process complexity increase
Solution Approach 1:
The patent applies preliminary action by pre-modifying the genetic structure of Trichoderma reesei strains to constitutively enhance protein production capability. This genetic preprocessing allows the organism to produce high levels of cellulase without requiring external induction by glucose or lactose addition during cultivation, thereby simplifying the manufacturing process while maintaining high productivity.
Solution Approach 2:
The patent enables self-service by creating mutant strains that inherently possess enhanced protein production capability through genetic modification. These self-sufficient strains can maintain high cellulase production levels without external supplementation of inducers like glucose or lactose, reducing manufacturing complexity and operational costs while sustaining high productivity.
Data Source
AI summary
A mutant strain of Trichoderma reesei, the mutant strain having a mutation that eliminates or reduces a function of a polypeptide consisting of the amino acid sequence represented by SEQ ID No: 6. A method produces a cellulase, the method including a step of cultivating the mutant strain of Trichoderma reesei, the mutant strain having a mutation that eliminates or reduces a function of a polypeptide consisting of the amino acid sequence represented by SEQ ID No: 6.