In Vitro Viability Detection for Trichuris suis Eggs
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Solution Overview
Problem
Current methods for determining the viability of Trichuris suis eggs are labor-intensive, requiring the breeding and slaughter of pigs, and are time-consuming, making them economically and practically challenging for standardized testing by semi-skilled personnel.
Innovation Solution
Simulating the gastrointestinal passage of a pig in vitro by exposing Trichuris suis eggs to a controlled environment with decreasing enzyme and salt concentrations, pH values, temperature, and gas concentrations, which mimics the stomach and intestinal environments, causing the larvae to hatch in a liquid or semi-solid solution.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If pigs are bred and slaughtered to examine Trichuris suis eggs, then viability detection is achieved, but great effort and time are required
Solution Approach 1:
The patent creates an in vitro model that copies the pig gastrointestinal environment using artificial stomach and intestinal fluids with appropriate pH values, enzyme concentrations, and incubation conditions. This artificial system replicates the biological processes needed for larval hatching without requiring actual pigs, thereby reducing time and effort while maintaining detection reliability
Solution Approach 2:
The patent replaces the mechanical/biological system of live pig digestion with a controlled chemical incubation system. By using defined enzyme concentrations, pH buffers, and temperature-controlled incubation, the complex biological process is substituted with a standardized laboratory procedure that is faster and more efficient
2Reliability
If pigs are bred and slaughtered to examine Trichuris suis eggs, then viability detection is achieved, but great effort in breeding and housing is required
Solution Approach 1:
The patent extracts the essential functional components of the pig gastrointestinal system (enzymes, pH conditions, temperature, incubation time) and isolates them in an artificial medium. This extraction eliminates the need for entire living animals, reducing process complexity while preserving the core functionality needed for viability detection
Solution Approach 2:
The patent defines specific parameter ranges for the artificial gastrointestinal fluids (pH 1.2-2.0 for stomach fluid, pH 6.8-7.4 for intestinal fluid, enzyme concentrations, incubation temperatures of 37-39°C). These controlled parameters standardize the process, making it easier to perform and reproduce without requiring complex animal husbandry
3Ease of operation
If standardized in vitro testing is performed, then ease of operation is improved, but simulation accuracy must be maintained
Solution Approach 1:
The patent divides the gastrointestinal passage into distinct segments (stomach environment with pH 1.2-2.0 and intestinal environment with pH 6.8-7.4), each with specific enzyme concentrations and incubation conditions. This segmentation allows semi-skilled personnel to perform standardized steps while maintaining the biological accuracy of each gastrointestinal region
Solution Approach 2:
The patent specifies precise parameter ranges (pH values, enzyme concentrations, temperatures, incubation times) that balance ease of operation with simulation accuracy. These defined parameters enable semi-skilled personnel to reproduce reliable results without requiring expert knowledge, while still maintaining faithful representation of the pig gastrointestinal environment
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method allows for the efficient and economical determination of egg viability in a controlled setting, reducing the need for animal breeding and slaughter, and can be performed by semi-skilled personnel, providing a standardized and rapid assessment of larval hatching.
Implementation Method 1
adding appropriate enzymes and salts and incubation at elevated temperature, whereby the following parameters are advantageously set: Enzymes, in the intestinal simulation with decreasing concentration
Implementation Method 2
exposing Trichuris suis eggs to a controlled environment with decreasing enzyme and salt concentrations, pH values, temperature, and gas concentrations
Implementation Method 3
incubation at elevated temperature, whereby the following parameters are advantageously set: heat
Data Source
AI summary
Method for detecting the viability of Trichuris suis eggs, characterized in that the passage of the eggs through the gastrointestinal passage of a pig is simulated in vitro with regard to the temporal sequence and environment, namely first of all the gastric environment and then, with decreasing enzyme and salt concentration, the intestinal environment, and the larvae are thus caused to hatch from the eggs.