Tripeptide Purification via Low pH Precipitation
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
The tripeptide of formula (I), Ac-Met(O2)-Val-Val-OH, is prone to oxidation and has an intense off-color due to impurities, making it difficult to purify using conventional methods, which limits its application in cosmetics and pharmaceuticals.
Innovation Solution
A method involving the use of very low pH (≤2.5) to form a precipitation of the tripeptide, allowing for its efficient separation and purification, resulting in a colorless form suitable for cosmetic and pharmaceutical compositions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If conventional purification methods are used for the tripeptide, then the purification process is simple, but the tripeptide retains intense off-color due to difficult-to-remove impurities
Solution Approach 1:
The patent applies parameter changes by adjusting the pH to very low levels (pH ≤ 2.5) during the purification process. This drastic change in the chemical environment enables selective precipitation of the tripeptide while leaving impurities in solution, achieving effective purification without requiring complex multi-step procedures or specialized equipment.
2Reliability
If the tripeptide is used in cosmetic compositions, then it provides anti-aging benefits, but its intense off-color severely limits its application
Solution Approach 1:
The patent converts the harmful off-color effect into a beneficial outcome by using the very property that causes the off-color (impurity association) against itself. At very low pH, the tripeptide precipitates in a pure form while impurities remain soluble, transforming the previously harmful coloration into a marker for successful purification.
3Ease of manufacture
If simple purification methods are used, then the process is economically favorable, but impurities are very difficult to remove
Solution Approach 1:
The patent achieves effective impurity removal through a single parameter change - adjusting pH to ≤ 2.5. This simple adjustment triggers selective precipitation of the tripeptide while impurities remain in the aqueous phase, enabling economical purification without requiring complex chromatography or multiple extraction steps.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method effectively removes impurities, producing a highly pure, colorless tripeptide that can be used in cosmetic and pharmaceutical compositions, overcoming previous limitations due to off-color issues.
Implementation Method 1
bringing the composition of step b) to a pH ≤ 2.5; forming a precipitation of the tripeptide of the formula (I)
Data Source
AI summary
The present invention relates to purification of a tripeptide of formula (I). By using said method, the tripeptide of formula (I) is obtained in a very pure form and due to this effect the purified tripeptide can be used in a variety of cosmetic and pharmaceutic compositions which are not accessible for the respective unpurified tripeptide.