Triple-Coated Lactic Acid Bacteria for Gastric Acid Resistance
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Solution Overview
Problem
Conventional methods for coating lactic acid bacteria are costly, difficult to sterilize, and do not provide adequate heat, acid, and bile resistance, leading to incomplete coating and reduced viability.
Innovation Solution
A method involving triple-coating lactic acid bacteria with protein, polysaccharides, and nanoparticles, specifically using solid lipid nanoparticles, to enhance stability and resistance.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional coating techniques using capsules or gelatin are used, then lactic acid bacteria are protected from gastric acid and bile acid, but production cost increases due to expensive coating agents and additional coating processes
Solution Approach 1:
The patent applies composite materials by combining multiple coating layers (protein coating, polysaccharide coating, and nanoparticle coating) to create a multi-functional protective structure. This composite approach provides superior protection against gastric acid and bile acid while optimizing the use of each material's properties to achieve effective protection without excessive cost
Solution Approach 2:
The coating process is segmented into three distinct stages: protein coating first, then polysaccharide coating, and finally nanoparticle coating. This segmentation allows each coating layer to perform its specific function optimally, with the protein providing base protection, polysaccharide adding structural integrity, and nanoparticles providing enhanced resistance, thereby achieving reliable protection through a systematic multi-step approach
2Reliability
If conventional coating techniques are used, then lactic acid bacteria are protected to some extent, but coating completeness is insufficient leading to inadequate heat resistance, acid resistance and bile resistance
Solution Approach 1:
The patent employs composite materials with three different coating layers, each contributing unique properties: protein provides base protection, polysaccharide adds structural integrity and moisture barrier, and nanoparticles provide enhanced resistance to heat, acid, and bile. This composite structure achieves superior coating completeness and multi-resistance properties that single or double coating methods cannot attain
Solution Approach 2:
The patent adds another dimension to the coating structure by introducing nanoparticle coating as a third layer. This dimensional addition (from single-layer to multi-layer to nano-layer) significantly enhances coating completeness and provides superior resistance properties, transforming the protective structure from basic to advanced through dimensional enhancement
3Ease of manufacture
If separate coating processes are used following bacterial collection, then coating can be applied, but aseptic manipulation becomes difficult and other bacteria may be included
Solution Approach 1:
The patent performs preliminary action by integrating the coating process with the bacterial culture process itself. The coating materials are added directly to the culture medium before bacterial growth, allowing the bacteria to be coated during cultivation rather than requiring separate post-collection coating steps. This preliminary integration maintains aseptic conditions throughout while achieving effective coating
4Reliability
If lyophilization with cryoprotectant and stabilizer is performed after coating, then superior viability and stability are ensured, but interactions between materials occur and processes become redundant
Solution Approach 1:
The patent merges the coating process with the stabilization process by incorporating cryoprotectant and stabilizer functions into the coating layers themselves. The protein, polysaccharide, and nanoparticle coatings simultaneously provide protective barriers and stabilize the bacteria, eliminating the need for separate lyophilization with additional cryoprotectants. This merging reduces process complexity while maintaining viability and stability
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The triple-coated lactic acid bacteria exhibit improved acid resistance, bile resistance, and stability, maintaining physiological activities even in acidic conditions and during storage.
Implementation Method 1
treating an aqueous protein solution with a protease
Implementation Method 2
adding glucose, yeast extract, meat extract, an ionic component and lactic acid bacteria to the aqueous protein solution enzyme-treated in the step (a), and fermenting to prepare single-coated lactic acid bacteria
Implementation Method 3
triple-coated lactic acid bacteria with improved bacterial stability and processing stability through prevention of direct interaction with air and moisture and through enhanced heat resistance, acid resistance and bile resistance
Data Source
Figure 1~2
AI summary
The present invention relates to a method for preparing triple-coated lactic acid bacteria, triple-coated lactic acid bacteria prepared thereby, and an article comprising the same. More particularly, the present invention relates to a method for preparing lactic acid bacteria triple-coated with protein, polysaccharide and nanoparticles, triple-coated lactic acid bacteria prepared thereby, and an article comprising the same. The triple-coated lactic acid bacteria prepared according to the present invention provides have better acid resistance, bile resistance and stability in accelerated test than conventional double-coated lactic acid bacteria. They are not killed by gastric acid or bile acid upon uptake, and maintain their inherent physiological activities, and, therefore, they can be effectively used in various products, including fermented milk, processed milk, fermented soybean products, fermented kimchi products, functional drinks, functional foods, conventional foods, cosmetics, and the like.