Triple-Parametric Optical Mapping for Cardiac Physiology
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current cardiac physiology studies lack the capability to simultaneously assess the interdependent facets of metabolic, electrical, and mechanical components, which are crucial for understanding drug effects and disease modulation, as existing methods primarily focus on electrical activity and contractility, neglecting calcium handling and metabolic states.
Innovation Solution
A triple-parametric optical mapping system using three cameras to simultaneously capture NADH, voltage (Vm), and Ca2+ signals from the same field of view, enabling the simultaneous measurement of up to ten physiological parameters, including action potential upstroke, calcium release, and metabolic state, to provide a comprehensive picture of cardiac physiology modulation by drugs or disease.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If multiple separate measurement methods are used for electrical activity, calcium handling, and metabolic state, then each parameter can be measured with sufficient precision, but the system complexity increases and simultaneous assessment becomes difficult
Solution Approach 1:
The patent combines three separate optical measurement systems into a single integrated optical mapping system that simultaneously measures voltage (Vm), calcium (Ca2+), and metabolic state (NADH) using multiple cameras and filter cubes. This merging approach maintains measurement precision for each parameter while reducing overall system complexity and enabling simultaneous assessment of all three cardiac physiological facets.
Solution Approach 2:
The optical mapping system is designed with multi-functionality to perform three distinct measurement functions simultaneously: voltage sensing, calcium imaging, and metabolic state assessment. The system uses a universal optical platform with multiple cameras and wavelength-specific filter cubes that can capture different physiological parameters through a single integrated apparatus, eliminating the need for separate measurement devices.
2Adaptability or versatility
If existing optical mapping methods are used, then electrical activity and some metabolic parameters can be recorded, but simultaneous assessment of all three interdependent facets (metabolic, electrical, and mechanical) is not achieved
Solution Approach 1:
The optical mapping system is segmented into three specialized measurement channels, each optimized for a specific physiological parameter: voltage sensing, calcium imaging, and metabolic state assessment. Each channel uses dedicated cameras and wavelength-specific filter cubes to capture its target parameter with high fidelity. This segmentation allows the system to simultaneously assess all three interdependent facets of cardiac function without cross-interference, preserving complete physiological information.
3Quantity of substance
If traditional electrical recording methods are used, then electrical activity can be measured, but metabolic state and calcium handling remain unassessed
Solution Approach 1:
The system merges electrical recording, calcium imaging, and metabolic sensing into a single optical mapping platform. By integrating multiple measurement modalities simultaneously, the system increases the quantity of physiological information obtained while managing device complexity through a unified optical architecture that uses coordinated cameras and filter cubes for each measurement type.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for the detailed analysis of cardiac physiology, identifying both on-target and off-target effects of drugs and the modulation of cardiac physiology during ischemia and reperfusion, providing valuable insights for drug testing and disease assessment, and offering a more comprehensive understanding of cardiac function than existing methods.
Implementation Method 1
optical mapping is a methodology that optically records cardiac physiology with high spatial and temporal resolution, either as autofluorescence of endogenous biological substances or as fluorescence of specifically designed dyes
Implementation Method 2
a first filter cube in the path of the light with a first light filter, where the first light filter directs filtered light to a first camera
Data Source
AI summary
Systems and methods are disclosed for an optical mapping device. The device emits different wavelengths of light from a plurality of light sources to a cardiac tissue and passes the light through a lens, a first filter cube in the path of the light with a first light filter, a second light filter, and a third light filter. Light passing through the filters is recorded by three cameras that each record an indicator of cardiac physiology, which are mapped simultaneously by the device.