Triple Vital Bacteria Composition Stability via Composite Protection
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Solution Overview
Problem
Existing triple vital bacteria compositions comprising Bifidobacterium longum, Lactobacillus acidophilus, and Streptococcus faecalis have poor stability, leading to low survival rates and limited long-term conservation, with existing protection agents and culture mediums not adequately addressing these issues.
Innovation Solution
A triple vital bacteria composition is developed using powders of Bifidobacterium longum, Lactobacillus acidophilus, and Streptococcus faecalis with specific protection agents and seed/fermentation culture mediums, including defatted milk powder, sodium glutamate, isolactose, Vc-Na, and starch, followed by freeze-drying to enhance stability and survival rates.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Duration of action of stationary object
If existing protection agents are used to preserve triple vital bacteria composition, then the bacteria can be preserved for some time, but the stability is poor and survival rate is low
Solution Approach 1:
The patent uses a composite protection agent system consisting of multiple components including skimmed milk powder, isolactose, starch, sodium glutamate, and Vc-Na. This composite formulation provides synergistic protection for the bacteria during freeze-drying and storage, significantly improving both the duration and reliability of bacterial preservation compared to single protection agents.
Solution Approach 2:
The patent optimizes the proportions of each protection agent component and adjusts freezing and drying parameters to achieve maximum bacterial survival. By changing physical and chemical parameters of the protection system, the patent resolves the contradiction between preservation duration and stability.
2Quantity of substance
If conventional culture mediums are used for Bifidobacterium, Lactobacillus acidophilus and Streptococcus faecalis, then the bacteria can be cultivated, but the stability and survival rate are insufficient
Solution Approach 1:
The patent develops separate optimized culture mediums for different bacterial strains with specific compositional requirements. Each culture medium is tailored to the local needs of specific bacteria (Bifidobacterium, Lactobacillus acidophilus, or Streptococcus faecalis), providing optimal growth conditions while ensuring high stability and survival rates for each strain.
3Reliability
If the bacteria are preserved without improved protection agents, then the formulation is simple, but the survival rate is low and long-term conservation is not achievable
Solution Approach 1:
The patent changes the chemical composition parameters of the protection agent system by incorporating specific ratios of skimmed milk powder, isolactose, starch, sodium glutamate, and Vc-Na. These parameter changes enable high survival rates and long-term conservation while maintaining a relatively simple formulation that can be easily manufactured.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The new composition significantly increases the stability and survival rate of the bacteria, allowing for longer conservation and improved efficacy in treating constipation and regulating intestinal flora.
Implementation Method 1
followed by freeze-drying to enhance stability and survival rates
Data Source
AI summary
The present invention provides a triple vital bacteria composition, including: powder of Bifidobacterium longum, powder of Lactobacillus acidophilus, and powder of Streptococcus faecalis. The present invention further provides a method for preparing the above triple vital bacteria composition, as well as the protection agents, the fermenting culture medium and the seed culture medium used therein.