trnG-trnS DNA Barcode for Wolfberry Species Identification
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Solution Overview
Problem
Traditional morphological identification methods are inadequate for distinguishing between Wolfberry (Lycium chinensis) species due to similarities in development morphology, tissue structure, and chemical composition, and the existing DNA barcode database for wolfberries is deficient in richness and quantity.
Innovation Solution
The development of a trnG-trnS DNA barcode system for rapid identification of Wolfberry species, including Lycium chinensis, which involves extracting genomic DNA, amplifying the trnG-trnS sequence, sequencing the PCR product, and building a phylogenetic tree to identify species and determine interspecies relationships.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If traditional morphological identification methods are used, then the identification process is simple and accessible, but the identification accuracy is insufficient due to similarities in development morphology, tissue structure, and chemical composition among wolfberry species
Solution Approach 1:
The patent replaces traditional morphological identification methods with DNA barcode sequencing technology. Specifically, it uses the trnG-trnS intergenic region sequence as a molecular marker to substitute for visual inspection of morphological characteristics, thereby achieving high-accuracy species identification that overcomes the limitations of similar appearance and tissue structure among wolfberry species
Solution Approach 2:
The patent introduces DNA sequence data as an intermediary element between the wolfberry samples and the identification result. By sequencing the trnG-trnS region and comparing it with reference databases, the method creates a reliable intermediary link that enables accurate differentiation of species that are morphologically similar
2Quantity of substance
If existing DNA barcode methods are used, then species identification can be achieved, but the barcode database is deficient in richness and quantity
Solution Approach 1:
The patent performs preliminary action by pre-sequencing and storing the trnG-trnS barcode sequences of multiple wolfberry species in a constructed database before actual identification needs arise. This advance preparation of reference data enables rapid comparison and identification without time-consuming database construction when samples need to be identified
Solution Approach 2:
The patent develops a universal trnG-trnS barcode system that can identify multiple wolfberry species simultaneously. The same barcode region and sequencing method can be applied across different species and research contexts, making the database construction effort universally applicable to various identification needs
Data Source
AI summary
The invention belongs to the technical field of species identification of Lycium chinensis, specially relating to a DNA barcode for identifying Lycium chinensis species and an identification method therefor. The DNA barcorde provide by the present invention can be applied to building a phylogenetic tree to be used for studying intra-species and inter-species phylogeny of Lycium chinensis, providing an effective basis for identification, classification and phylogenetic study of Lycium chinensis. The invention also provides a trnG-trnS barcode database, to effectively identify the species of the Lycium chinensis and determine the interspecies relationship of Lycium chinensis, thereby providing an effective basis for the species of Lycium chinensis.