Tumour-Selective CO Conjugates for Local Cancer Immunomodulation

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Solution Overview

Problem

Existing immunotherapies for cancer are not effective in all patients and do not yield complete or durable clinical responses, and the role of carbon monoxide (CO) in cancer immunity is not fully understood, necessitating a need for controlled and specific delivery of CO to target tumour tissues to reduce immunosuppression.

Innovation Solution

Development of CO releasing-conjugates comprising tumour-selective ligands, such as human serum albumin, that selectively accumulate in tumour tissue, releasing CO to downregulate immune checkpoint molecules and reduce tumour-associated macrophages, thereby reducing immunosuppression.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If CO-releasing molecules are used to treat cancer, then anti-tumour effects are observed, but CO may release in circulation causing toxicity

Engineering Contradiction:
Improveanti-tumour effectVSAvoidCO toxicity in circulation
Core Design Contradiction:
ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent uses tumour-selective ligands as intermediaries to deliver CO-releasing molecules specifically to tumour tissues. The ligand acts as a mediator that binds to the CORM and directs it to the tumour site through selective accumulation, preventing premature CO release in circulation while ensuring therapeutic CO delivery at the target site.

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent creates local selectivity by conjugating CORMs with tumour-selective ligands that accumulate specifically in tumour tissue. This ensures that CO is released locally at the tumour site rather than systemically, achieving high local concentration for therapeutic effect while maintaining low systemic concentration to avoid toxicity.

Inventive Principle:
Principle #3Local quality

2Reliability

If conventional immunotherapies are used, then immune response is activated, but they do not yield complete or durable clinical responses

Engineering Contradiction:
Improveimmune response activationVSAvoiddurability of clinical response
Core Design Contradiction:
ReliabilityVSDuration of action of stationary object

Solution Approach 1:

The patent converts the immunosuppressive tumour microenvironment, which normally protects cancer cells, into a therapeutic target. By delivering CO specifically to tumour tissue, the treatment exploits the tumour's own immunosuppressive properties to concentrate the therapeutic agent where it is most needed, while simultaneously reversing the immunosuppression to enhance immune-mediated tumour destruction.

Inventive Principle:
Principle #22Blessing in disguise (Convert harm into benefit)

3Quantity of substance

If CORMs are administered systemically, then higher therapeutic doses can be given, but CO release in circulation occurs causing poisoning

Engineering Contradiction:
Improvetherapeutic dose of COVSAvoidCO poisoning
Core Design Contradiction:
Quantity of substanceVSObject-affected harmful factors

Solution Approach 1:

The patent achieves local quality by creating tumour-targeted CORM conjugates that selectively accumulate in tumour tissue. This allows administration of higher doses of CO-releasing molecules because the CO is released primarily at the tumour site rather than throughout the circulation, maintaining a favourable therapeutic index.

Inventive Principle:
Principle #3Local quality

Data Source

PatentEP4126052B1Use of conjugates comprising tumour-selective ligands and groups capable of releasing carbon monoxide (CO), for exerting immunomodulatory effects in cancer treatment
Publication Date: 2025.12.24 FUNDACAO GIMM GULBENKIAN INSTITUTE FORMOLECULAR MEDICINE
  • EP4126052B1 patent drawingFigure 1a~1b
  • EP4126052B1 patent drawingFigure 2a~2b
  • EP4126052B1 patent drawingFigure 3

AI summary

The present invention in various aspects and embodiments involves the use of CO releasing molecules, such as conjugates comprising tumour-selective ligands and groups capable of releasing carbon monoxide (CO), for exerting immunomodulatory effects in cancer treatment, such as reductions in the expression of immune checkpoint molecules and the level of inhibitory macrophages. This can reduce immunosuppression in tumour tissue and can be useful, for example, in the treatment of immunosuppressive cancers or the treatment of cancers in combination with cancer immunotherapy.