Tv40Srp PCR Detection for Specific Trichomonas Vaginalis Testing

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Solution Overview

Problem

Current methods for detecting Trichomonas vaginalis (TV) are not highly specific, accurate, or sensitive, particularly in urine or swab-based tests.

Innovation Solution

The development of a method to detect the TV 40S ribosomal protein (Tv40Srp) gene or RNA in a sample using PCR, which includes the use of specific primers and probes to enhance specificity and sensitivity.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If conventional detection methods are used for Trichomonas vaginalis, then the testing process is simpler, but the specificity, accuracy, and sensitivity are insufficient

Engineering Contradiction:

Improvedetection accuracyVSAvoidtesting complexity

Core Design Contradiction:

Measurement precisionVSDevice complexity

Solution Approach 1:

The patent replaces conventional mechanical or chemical detection methods with a molecular biological detection system based on PCR technology. This substitution enables highly specific detection of Tv40Srp gene sequences, achieving superior accuracy and sensitivity while maintaining a streamlined testing workflow through automated thermal cycling and fluorescent detection.

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

2Reliability

If PCR method with specific primers and probes is used, then the specificity and sensitivity are improved, but the testing time and complexity increase

Engineering Contradiction:

Improvedetection reliabilityVSAvoidtesting time

Core Design Contradiction:

ReliabilityVSLoss of time

Solution Approach 1:

The patent incorporates preliminary actions by designing specific primers and probes that pre-target the Tv40Srp gene sequence, and by including internal controls in the reaction mixture beforehand. This preparation enables the PCR reaction to proceed efficiently with rapid amplification and detection, reducing overall testing time while ensuring reliable results through built-in validation mechanisms.

Inventive Principle:

Principle #10Preliminary action

3Measurement precision

If PCR method is used to detect TV gene or RNA, then the detection sensitivity is improved, but the device and procedure complexity increases

Engineering Contradiction:

Improvedetection sensitivityVSAvoidprocedure complexity

Core Design Contradiction:

Measurement precisionVSDevice complexity

Solution Approach 1:

The patent employs a universal PCR detection system that can detect both DNA and RNA targets (through reverse transcription) using the same basic apparatus and protocol framework. The method uses universal reagents and procedures that work across different sample types, reducing the need for multiple specialized devices and procedures while maintaining high detection sensitivity for the Tv40Srp gene.

Inventive Principle:

Principle #6Universality (Multi-functionality)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This method provides a highly specific, accurate, and sensitive means to detect TV in samples, allowing for rapid and reliable diagnosis.

Implementation Method 1

The development of a method to detect the TV 40S ribosomal protein (Tv40Srp) gene or RNA in a sample using PCR

Methodology Applied

Scientific EffectPCR (Polymerase Chain Reaction):

Implementation Method 2

which includes the use of specific primers and probes to enhance specificity and sensitivity

Methodology Applied

Scientific EffectHybridization:

Data Source

PatentUS20250290160A1Methods of Detecting Trichomonas Vaginalis

Publication Date: 2025.09.18 CEPHEID INC

AI summary

Compositions and methods for detecting Trichomonas vaginalis are provided.