Two-Part Cuvette Coating Composition for Clear Buffy Coat Imaging
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Solution Overview
Problem
Existing cuvette compositions for biological sample analysis, particularly for blood samples, fail to provide a clear image of the buffy coat layer, and there is a need for improved compositions that facilitate easy sample collection, processing, and imaging.
Innovation Solution
A composition for coating cuvettes comprising a collection solution with anticoagulant, agglutination, and wetting agents, and an imaging solution with acridine orange, which are applied separately to form a two-part coating that enhances sample uptake, maintains sample integrity, and improves imaging clarity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If a traditional single composition is used to coat the cuvette, then the cuvette can be manufactured at low cost, but the image clarity of the buffy coat layer is poor
Solution Approach 1:
The coating composition is divided into two separate solutions: a first solution containing anticoagulant and agglutination agents for sample collection, and a second solution containing imaging agents for clear visualization. This segmentation allows each solution to be optimized for its specific function, improving image clarity while maintaining manageable complexity through separate formulation and application steps.
Solution Approach 2:
Different regions of the cuvette are coated with different compositions: the first solution is applied to regions requiring sample collection assistance, while the second solution is applied to regions requiring clear imaging. This local differentiation ensures optimal performance for each functional requirement without compromising overall system complexity.
2Productivity
If a single coating composition is applied to the cuvette, then the coating process is simple, but the sample collection efficiency and imaging quality cannot be simultaneously optimized
Solution Approach 1:
The coating process is segmented into two separate applications: first applying the collection solution with anticoagulant and agglutination agents to enhance sample uptake, then applying the imaging solution with imaging agents to ensure clear visualization. This segmentation enables simultaneous optimization of collection efficiency and imaging quality while maintaining a straightforward two-step process.
Solution Approach 2:
The first coating solution is applied in advance to prepare the cuvette surface for efficient sample collection, creating an optimized environment for sample uptake before the imaging step. This preliminary action ensures that sample collection efficiency is maximized before the imaging process begins.
3Measurement precision
If conventional cuvette coating compositions are used, then the cuvette is easy to manufacture, but the buffy coat layer cannot be visualized clearly
Solution Approach 1:
The coating composition is segmented into two separately manufacturable solutions: the first solution with anticoagulant and agglutination agents for sample collection, and the second solution with imaging agents for clear buffy coat visualization. This segmentation enables optimized formulation for each function while maintaining ease of manufacture through independent preparation and quality control processes.
Solution Approach 2:
The composition parameters are optimized independently for each solution: the first solution uses specific concentrations of anticoagulant and agglutination agents for efficient sample collection, while the second solution uses optimized imaging agent concentrations for clear buffy coat visualization. This parameter optimization maintains manufacturing ease while significantly improving measurement precision.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The composition allows for efficient collection and clear imaging of the buffy coat layer in blood samples, reducing variability and ensuring effective visualization of different blood cell layers during analysis.
Implementation Method 1
the collection solution comprises: an anticoagulant agent
Implementation Method 2
an agglutination agent
Implementation Method 3
a wetting agent
Implementation Method 4
an imaging solution with acridine orange
Data Source
AI summary
The present invention relates to a composition and a method of making a composition. In particular, the present invention relates to a composition for coating a cuvette and a method of making a composition for coating a cuvette.