Two-Plate NMR Drop Analyzer for Microliter Samples

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Solution Overview

Problem

Conventional NMR spectroscopy is limited by the requirement for large sample volumes, which is not suitable for small samples commonly found in life sciences, hindering miniaturization and increasing the need for precise analysis of microliter samples.

Innovation Solution

A device utilizing a mechanism with two plates to hold a sample as a drop between them, allowing for a defined distance and increased contact area, combined with a sensor unit to detect nuclear magnetic resonances, enabling analysis of volumes less than 100 µl.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If conventional NMR spectroscopy with induction coils and strong static magnetic fields is used, then measurement precision is improved, but device complexity increases and miniaturization is precluded

Engineering Contradiction:
Improvedetection sensitivityVSAvoidsystem complexity
Core Design Contradiction:
Measurement precisionVSDevice complexity

Solution Approach 1:

The patent extracts the essential function of magnetic field sensing from complex induction coils and strong static field generators, replacing them with a simplified sensor unit based on nitrogen vacancy centers in diamond that can detect magnetic fields without requiring strong polarizing fields or large induction coils

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The patent replaces the mechanical and electromagnetic system of induction coils and strong magnetic fields with a quantum sensor system based on optical detection of nitrogen vacancy centers, substituting complex electromagnetic mechanisms with quantum optical effects

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Measurement precision

If strong static magnetic fields are used to polarize nuclear spins, then measurement precision is improved, but the requirement for large sample volumes increases

Engineering Contradiction:
Improvesignal strengthVSAvoidsample volume
Core Design Contradiction:
Measurement precisionVSQuantity of substance

Solution Approach 1:

The patent changes the detection parameter from requiring strong magnetic field polarization to detecting magnetic field variations directly through quantum sensor response, enabling sensitive detection with minimal sample volumes

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent uses composite sensor structures combining diamond with nitrogen vacancy centers and optical detection systems to achieve high sensitivity with minimal sample requirements

Inventive Principle:
Principle #40Composite materials

3Measurement precision

If the sample is placed in a long glass tube as in conventional NMR, then measurement precision is improved, but the device complexity and sample volume requirement increase

Engineering Contradiction:
Improvespectral resolutionVSAvoidsample holder complexity
Core Design Contradiction:
Measurement precisionVSDevice complexity

Solution Approach 1:

The patent removes the long glass tube sample holder from the system, replacing it with a simplified sample placement area between two plates where the sample can be applied directly as a drop or thin layer

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The patent uses thin plate structures instead of long glass tubes, creating a compact sample chamber that accommodates minimal sample volumes while maintaining detection precision

Inventive Principle:
Principle #30Flexible shells and thin films

4Device complexity

If conventional NMR instruments are miniaturized, then device complexity is reduced, but measurement precision deteriorates

Engineering Contradiction:
Improveinstrument sizeVSAvoiddetection sensitivity
Core Design Contradiction:
Device complexityVSMeasurement precision

Solution Approach 1:

The patent replaces the mechanical miniaturization approach with quantum sensor-based detection that inherently provides high sensitivity in compact form factors, using optical detection of quantum states rather than scaled-down electromagnetic components

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent changes the fundamental detection parameter to quantum optical signals from nitrogen vacancy centers, which provide high sensitivity without requiring the large component sizes needed for conventional NMR

Inventive Principle:
Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Facilitates the detection of nuclear magnetic resonances in small sample volumes by enhancing sensitivity and preventing evaporation, while allowing easy loading and cleaning.

Implementation Method 1

a sensor unit (11) with a sensor component (12) which forms at least a partial region of the first plate (5) and/or the second plate (6) and is at least partially in contact with the sample (4), wherein the sensor unit (11) is designed to detect a quantity influenced by the nuclear magnetic resonances of the sample (4)

Methodology Applied
Scientific EffectNuclear magnetic resonance: Magnetic Field

Data Source

PatentEP4384839B1Device for analyzing a liquid or pasty sample, which is provided in the form of drops, using nuclear magnetic resonances of the sample
Publication Date: 2025.09.03 ANALYTIK JENA GMBHCO KG
  • EP4384839B1 patent drawingFigure 1~2
  • EP4384839B1 patent drawingFigure 3~4

AI summary

The invention relates to a device (3) for analyzing a liquid or pasty sample (4), which is provided in the form of drops, using nuclear magnetic resonances of the sample (4), comprising - a first plate (5) and a second plate (6), - a mechanism (7) via which a measuring position (8) and a recording position (9) can be set, wherein the first plate (5) and the second plate (6) are substantially parallel to each other in the measuring position (8), and a spacer (10) sets a defined spacing between the first plate (5) and the second plate (6), - a sensor unit (11) with a sensor component (12) which forms at least one sub-region of the first plate (5) and/or the second plate (6) and at least partly contacts the sample (4), said sensor unit (11) being designed to detect a variable which is influenced by the nuclear magnetic resonances of the sample (4), and - an analysis unit (13).