Tyrosine Oxide Biomaterial Protein Detection
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Solution Overview
Problem
Current methods for diagnosing diseases, such as histological analysis, are invasive, complex, and not suitable for rapid real-time diagnosis, and existing bio-imaging techniques face issues with biocompatibility and practicality for use in operating rooms.
Innovation Solution
A composition containing tyrosine oxide-coupled biomaterials that bind to proteins, allowing for easy and rapid protein detection using a dot blotting method, enabling real-time disease diagnosis in an operating room without the need for secondary antibody reactions or complex staining processes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional histological analysis is used for disease diagnosis, then high accuracy and reproducibility are achieved, but the process is complex, invasive, and cannot provide real-time results
Solution Approach 1:
The patent uses tyrosine oxide-coupled biomaterials that produce visible color changes when they bind to target proteins in tissue. This colorimetric detection method eliminates the need for complex staining procedures while maintaining high detection accuracy, allowing real-time visual assessment of protein expression patterns during surgery.
Solution Approach 2:
The invention extracts and utilizes the natural pigment tyrosine oxide and its coupling properties with biomaterials to create a simplified detection system. By focusing on this specific biochemical interaction, the complex histological analysis process is reduced to a straightforward color-based detection method that can be performed rapidly on fresh tissue samples.
2Loss of time
If bio-imaging equipment such as PET or CT is used for direct tissue observation, then real-time diagnosis is enabled, but biocompatibility issues and requirement for external energy supply arise
Solution Approach 1:
The tyrosine oxide-coupled biomaterials are applied directly to fresh tissue sections and self-develop through natural color changes upon binding to target proteins. This eliminates the need for external energy sources, complex imaging equipment, or prolonged incubation periods, enabling rapid diagnosis within minutes while maintaining full biocompatibility with human tissue.
3Difficulty of detecting and measuring
If quantum dot or fluorescent material-coupled antibodies are used for tissue staining, then detection capability is improved, but the materials are synthetic and lack proven stability in human body
Solution Approach 1:
The patent transitions from using synthetic fluorescent materials to utilizing tyrosine oxide, a natural pigment with proven biocompatibility. By changing the detection parameter from fluorescence emission to visible color changes, the method maintains high detection capability while ensuring material stability and safety for potential clinical applications in human patients.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables rapid and safe real-time disease diagnosis in an operating room by visually identifying protein-bound tyrosine oxide-coupled biomaterials, effectively replacing conventional histological analysis with high specificity and simplicity.
Implementation Method 1
the tyrosine oxide-coupled biomaterial is bound to a protein to allow the color of the protein to be easily ascertained with the naked eye or an optical microscope
Data Source
AI summary
Provided is a composition for detecting a protein, containing a tyrosine oxide-coupled biomaterial. Various diseases may be easily and rapidly diagnosed by easily detecting the composition containing a tyrosine oxide-coupled biomaterial according to the present invention, by identifying a color of the protein bound with a tyrosine oxide-coupled biomaterial prepared by binding tyrosine oxide, which is a natural pigment present in a living body, with the biomaterial. Accordingly, the composition of the present invention may be used to easily and rapidly diagnose various diseases in real time in an operating room, and may usefully replace conventional histological analysis without a secondary antibody reaction and a final operation of color expression.