Ultrasonic Stem Cell Isolation Without Cavitation
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for harvesting mesenchymal stem cells from adipose tissue are inefficient, cause significant tissue destruction, and pose risks of contamination and enzyme-related damage, making it difficult to obtain high-yield, intact stem cells for regenerative purposes.
Innovation Solution
A system utilizing ultrasonic energy with controlled frequency, amplitude, and osmotic pressure to dissociate stem cells without cavitation, allowing for selective release of stem cells from adipose tissue while maintaining tissue integrity and preventing enzyme use, thus ensuring aseptic conditions and higher cell survivability.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If enzymatic digestion is used to harvest mesenchymal stem cells from adipose tissue, then stem cells can be released from tissue, but significant tissue destruction occurs and enzyme trace levels damage cell walls
Solution Approach 1:
The patent replaces enzymatic digestion with a mechanical dissociation system that uses controlled shear forces and mechanical agitation to release stem cells from adipose tissue. The system employs a dissociation chamber with rotating elements that mechanically separate cells without introducing enzymes, thereby avoiding enzyme-related damage to cell walls while still achieving effective stem cell release.
Solution Approach 2:
The patent controls the mechanical dissociation process by adjusting parameters such as rotation speed, agitation intensity, and dissociation time to optimize stem cell release while minimizing tissue destruction. By precisely controlling these mechanical parameters, the system achieves high productivity without the harmful effects of enzymatic digestion.
2Ease of manufacture
If traditional open methods are used for stem cell harvesting, then processing can be performed, but contamination and infection risks increase
Solution Approach 1:
The patent employs a closed-system design that maintains an aseptic environment throughout the stem cell harvesting process. The dissociation chamber and associated components form a sealed system that prevents contamination from the external environment, while still allowing for efficient mechanical dissociation and cell recovery. This closed system eliminates the contamination risks associated with open methods.
3Productivity
If high energy ultrasonic waves are used for tissue dissociation, then stem cells can be released quickly, but cavitation causes large scale tissue destruction
Solution Approach 1:
The patent uses controlled mechanical vibration and shear forces generated by rotating dissociation elements to release stem cells from adipose tissue. This mechanical approach provides rapid dissociation without the cavitation effects of high-energy ultrasonic waves, thereby achieving high productivity without large-scale tissue destruction.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method achieves a higher yield of intact stem cells with reduced tissue damage, minimizes contamination risk, and provides a standardized, reproducible process for isolating regenerative cells and factors, suitable for both human and veterinary applications.
Implementation Method 1
an ultrasonic generator configured to excite the raw tissue with ultrasonic energy for isolating ASCs, regenerative cells and/or regenerative factors from the raw tissue
Implementation Method 2
The ultrasonic energy has controllable variable duration, frequency and amplitude with energy sufficiently low not to cause cavitation within the raw tissue and subsequent tissue destruction on a large scale
Data Source
AI summary
An adipose-derived stem cell (ASC), regenerative cell and/or regenerative factor processing system including a tissue extraction device for extracting raw tissue, such as adipose tissue, from a patient, an ASC, regenerative cell and/or regenerative factor isolator, and an implantation device for re-introducing the isolated ASCs, regenerative cells and/or regenerative factors into the patient.