Filter Resuspension Stack Using Ultrasound for Multi-Sample Recovery
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Solution Overview
Problem
Existing techniques for resuspending biological material captured on filters are inefficient, requiring manual manipulation that can lead to loss and degradation of the material, and are not adaptable for simultaneous processing of multiple filters, leading to time constraints in obtaining laboratory results.
Innovation Solution
A device comprising a housing with a stack of fluidic distribution plates and filters, equipped with ultrasonic vibration, allows for simultaneous resuspension of biological material on multiple filters using a small volume of resuspension solution, ensuring effective detachment and recovery.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If manual manipulation is used to detach bacteria from filters, then resuspension can be performed, but biological material is lost and degraded
Solution Approach 1:
The patent replaces manual mechanical manipulation with an automated ultrasonic vibration system. The ultrasonic transducer generates high-frequency vibrations that mechanically detach bacteria from the filter surface without requiring manual handling, thus preventing biological material loss and degradation while maintaining ease of operation.
Solution Approach 2:
The patent employs ultrasonic vibration as the core mechanism to detach biological material from the filter. The ultrasonic transducer generates high-frequency mechanical vibrations that propagate through the resuspension solution, effectively detaching bacteria from the filter surface and suspending them in the solution without manual intervention.
2Loss of substance
If manual resuspension is performed on single filter, then material recovery is achieved, but processing time increases and multiple filters cannot be processed simultaneously
Solution Approach 1:
The patent merges multiple filter processing operations into a single integrated system. Multiple filters are positioned within the same housing and subjected to simultaneous ultrasonic vibration and resuspension, allowing parallel processing of multiple filters and significantly reducing total processing time while maintaining effective material recovery.
Solution Approach 2:
The patent designs a universal resuspension system that can simultaneously process multiple filters of the same type. The housing, ultrasonic transducer, and fluidic system are configured to accommodate multiple filters, enabling one system to perform multiple processing functions in parallel, thus reducing time loss.
3Loss of substance
If large volume of resuspension solution is used, then complete resuspension is achieved, but resource consumption increases
Solution Approach 1:
The patent uses ultrasonic vibration to enhance the efficiency of resuspension. The high-frequency mechanical vibrations create strong mixing and detachment forces that enable complete resuspension of biological material using a smaller volume of resuspension solution, reducing resource consumption while maintaining resuspension completeness.
Solution Approach 2:
The patent changes the physical parameters of the resuspension process by introducing ultrasonic vibration frequency and amplitude as key parameters. These parameter changes enable more efficient mass transfer and detachment, allowing complete resuspension with reduced solution volume compared to conventional static resuspension methods.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Facilitates rapid and efficient resuspension of biological material on multiple filters, reducing the risk of loss and degradation, and enabling rapid decision-making by providing results within hours.
Implementation Method 1
the case has an opening on its lower wall and/or on its upper wall to accommodate a head of an ultrasonic source
Data Source
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AI summary
The invention relates to a device used for the resuspension of biological material, comprising: - A housing having at least two parts assembled together in a hermetically sealed manner, said housing having several walls, these walls being composed of an upper wall, a lower wall and a side wall, said walls delimiting an internal volume, - A stack (E) made along a principal axis and placed in said internal volume and composed, along this principal axis, of at least a first assembly formed of a first fluidic distribution plate (40) and a first superimposed filter (30), said first fluidic distribution plate (40) having on its so-called upper face, oriented towards a face of the filter, several raised elements forming fluidic flow paths (50), - At least one fluidic inlet (IN) and one fluidic outlet (OUT) opening into said internal volume (V),opposite said fluid flow paths (50).