Umbilical Cord Extract for Regenerative Therapy
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Solution Overview
Problem
Current methods for promoting healing and therapeutic effects in humans and animals are inefficient and lack a straightforward, cost-effective approach for mass production and application of regenerative agents.
Innovation Solution
A method involving the extraction of proteins, cytokines, and growth factors from umbilical cord tissue and Wharton's jelly, using sonication and centrifugation to create a therapeutic and regenerative product that can be administered topically, systemically, or as a cell culture additive, enhancing healing and reducing inflammation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional methods are used for producing regenerative agents, then therapeutic effects can be achieved, but the production process is complex, expensive, and inefficient
Solution Approach 1:
The patent extracts and utilizes proteins, cytokines, and growth factors directly from umbilical cord tissue and Wharton's jelly through simple sonication and centrifugation, eliminating the need for complex cell culture systems and multiple purification steps while maintaining therapeutic efficacy
Solution Approach 2:
The patent changes the physical state and concentration parameters of the extract by adjusting sonication power, centrifugation speed, and filtration parameters to optimize the therapeutic composition while simplifying the production process
2Reliability
If complex extraction methods are used to obtain high concentrations of active ingredients, then therapeutic efficacy is improved, but production cost and time increase
Solution Approach 1:
The patent performs preliminary enrichment of proteins, cytokines, and growth factors in the umbilical cord tissue itself before extraction, allowing for high concentrations of active ingredients to be obtained through simple sonication and centrifugation without time-consuming multiple purification steps
Solution Approach 2:
The patent replaces complex biochemical purification systems with simple mechanical processes (sonication and centrifugation) to efficiently extract therapeutic components, dramatically reducing production time while maintaining high concentrations of active ingredients
3Manufacturing precision
If advanced purification techniques are applied to ensure product quality, then purity is improved, but manufacturing complexity and cost increase
Solution Approach 1:
The patent selectively extracts proteins, cytokines, and growth factors from umbilical cord tissue through sonication and centrifugation, separating therapeutic components from cellular debris and other contaminants to achieve high purity without complex purification techniques
Solution Approach 2:
The patent creates a locally optimized extraction environment by adjusting sonication parameters and centrifugation conditions to selectively isolate therapeutic components while leaving contaminants behind, achieving high purity through localized process optimization rather than multiple global purification steps
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method produces a product that effectively promotes healing, reduces inflammation, and treats various afflictions in mammals, with demonstrated efficacy in wound healing, muscle regeneration, and pain relief, offering a simple, inexpensive, and quick solution for mass production and application.
Implementation Method 1
applying an ultrasonic processer to the aqueous solution to sonicate and disrupt umbilical cord tissue
Implementation Method 2
placing the aqueous solution into a centrifuge to separate the aqueous solution into a soluble component and a non-soluble component
Data Source
AI summary
A method of preparation of a solution used for therapeutic and regenerative applications having a concentration of at least about 0.05 microgram of total protein extract is disclosed. The method includes collecting an umbilical cord, incubating the umbilical cord in a solution containing antibiotics for sterilization, washing the umbilical cord in a buffered salt solution, homogenizing the umbilical cord in the buffered salt solution so as to produce an aqueous solution, applying an ultrasonic processer to the aqueous solution to sonicate and disrupt umbilical cord tissue, placing the aqueous solution into a centrifuge to separate the aqueous solution into a soluble component and a non-soluble component, filtrating and discarding the non-soluble component of the aqueous solution from the centrifuge through a 0.2 micron filter, and measuring protein concentration of the soluble component to insure at least about 0.05 microgram of total protein extract.