Unified Signal Processing for MPEF Fluorescence Detection
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Solution Overview
Problem
MPEF microscopes require different architectures to measure fluorescence of varying intensities, leading to increased costs and inefficiencies due to the need for multiple instrument setups.
Innovation Solution
A signal processing system comprising an optical detector, analog signal processing module, and digital signal processing module that generates appropriate voltage signals from photocurrent signals, allowing for image generation based on signal strength, thereby standardizing the measurement process across different fluorescence intensities.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If different MPEF microscope systems with different architectures are used to measure fluorescence of varying intensities, then measurement accuracy is improved, but device complexity and cost increase
Solution Approach 1:
The patent implements a unified MPEF microscope system that can handle both strong and weak fluorescence signals through a single architecture. The system uses two parallel signal processing paths: an analog signal processing module for strong signals and a digital signal processing module for weak signals. This multi-functional approach allows one system to perform multiple measurement functions across different signal intensity ranges, eliminating the need for multiple specialized systems while maintaining measurement accuracy.
Solution Approach 2:
The system dynamically changes processing parameters based on signal intensity. When strong fluorescence signals are detected, the analog processing path is activated with specific gain and integration parameters. When weak signals are detected, the system switches to digital processing path with different parameters including noise filtering settings and integration time. This parameter adaptation allows optimal measurement accuracy across varying fluorescence intensities without requiring multiple fixed-architecture systems.
2Reliability
If multiple instrument architectures are deployed to handle varying fluorescence intensities, then measurement reliability is improved, but system cost increases
Solution Approach 1:
The patent merges previously separate measurement capabilities into a single integrated system. By combining analog signal processing and digital signal processing modules within one MPEF microscope, the system achieves the reliability of multiple specialized instruments while reducing the total quantity of equipment needed. The merged system includes shared components such as the excitation source, objective lens, and detector, with signal processing divergence occurring only at the appropriate stage.
Solution Approach 2:
The patent introduces an intermediary signal routing mechanism that directs strong signals to analog processing and weak signals to digital processing. This intermediary component acts as a mediator between the detector and the two processing paths, automatically selecting the appropriate processing mode based on signal intensity. This intermediary structure enables a single system to reliably handle diverse signal conditions without requiring multiple independent instrument architectures.
3Device complexity
If a single standardized microscope system is used for all fluorescence intensities, then device complexity is reduced, but measurement precision deteriorates
Solution Approach 1:
The patent segments the signal processing function into two distinct modules: analog signal processing and digital signal processing. Each segment is optimized for specific signal intensity ranges - analog processing for strong signals and digital processing for weak signals. This segmentation allows the single standardized system to maintain measurement precision across different intensity ranges by directing signals to the appropriate processing segment, avoiding the precision loss that would occur with a single unsegmented processing path.
Solution Approach 2:
The system dynamically switches between analog and digital processing modes based on real-time signal intensity assessment. This dynamic adaptability allows the standardized microscope system to optimize its processing path for each measurement condition, maintaining high measurement precision across varying fluorescence intensities. The dynamic switching mechanism ensures that each signal type is processed by the most appropriate module, preventing precision deterioration despite the use of a single standardized architecture.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables efficient and cost-effective fluorescence measurement by adapting signal processing to signal strength, reducing the need for multiple instrument architectures and lowering overall system costs.
Implementation Method 1
at least one optical detector configured for receiving an optical signal emitted from one of a plurality of positions-to-be-detected of an object-to-be-detected and generating a photocurrent signal
Data Source
AI summary
A signal processing method and a signal processing system are provided to convert optical or electric signals by an effective circuit to have an increased dynamic contrast and reduced noises. The signal processing system includes an analog signal processing module and a digital signal processing module. When an optical signal of an object-to-be-detected is strong, an image-to-be-detected is obtained by an analog signal processing method. When the optical signal of the object-to-be-detected is weak, the image-to-be-detected is obtained by a digital signal processing method after background noises are filtered out.