Uniform Coated Vessel for Embryoid Body Formation
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Solution Overview
Problem
Vessels for embryoid body formation coated with a polymer having a phosphorylcholine-like group suffer from nonuniform cell adhesion properties and optical observation issues due to coating thickness and pattern formation, affecting embryoid body formation and microscopic observation.
Innovation Solution
A method involving coating the inner surface of a vessel with a specific amount of a compound containing a phosphorylcholine-like group, followed by drying and leveling with a water/alcohol-based medium solution, and subsequent sterilization to create a homogeneous surface for efficient embryoid body formation and optical observability.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If a vessel is coated with a polymer having a phosphorylcholine-like group to prevent cell adhesion, then cell adhesion property is improved, but coating uniformity deteriorates causing nonuniform cell adhesion and optical observation issues
Solution Approach 1:
The patent changes the chemical composition parameters of the coating solution by adding a surfactant (0.01-1% by weight) to the phosphorylcholine-containing polymer solution. This parameter modification prevents coating film formation and ensures uniform distribution of the polymer on the vessel surface, resolving the contradiction between achieving reliable cell adhesion prevention and maintaining coating uniformity.
Solution Approach 2:
The patent introduces a surfactant as an intermediary substance that mediates between the phosphorylcholine-containing polymer and the vessel surface. The surfactant prevents polymer aggregation and coating film formation during the drying process, ensuring uniform coating while maintaining the anti-adhesion properties of the phosphorylcholine groups.
2Reliability
If the coating film thickness is increased to enhance cell adhesion prevention, then cell adhesion property is improved, but optical observation quality deteriorates due to pattern formation on the vessel surface
Solution Approach 1:
The patent modifies the concentration parameters of the coating solution by incorporating a surfactant at 0.01-1% by weight. This prevents the formation of thick, patterned coating films while maintaining sufficient phosphorylcholine groups on the surface for effective cell adhesion prevention, thus preserving optical observation quality.
Solution Approach 2:
The surfactant acts as an intermediary that prevents polymer aggregation during coating application. It ensures that even at higher polymer concentrations, the coating remains thin and uniform without forming visible patterns, allowing both effective cell adhesion prevention and high-quality optical observation.
3Reliability
If a coating solution is applied and dried to form a coating film, then cell adhesion prevention is achieved, but coating film formation causes nonuniformity and optical observation issues
Solution Approach 1:
The patent converts the harmful effect of coating film formation (which causes nonuniformity) into a benefit by using the drying process to evaporate the solvent while the surfactant prevents polymer aggregation. The result is a uniform distribution of phosphorylcholine groups on the vessel surface without visible coating patterns, achieving both cell adhesion prevention and coating uniformity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method produces a vessel with a uniform surface, enhancing the efficiency of embryoid body formation and optical observability, preventing cell adhesion and improving microscopic visibility.
Implementation Method 1
adding a water/alcohol-based medium solution to swell the coating film
Data Source
AI summary
An object is to provide a method of manufacturing a vessel for embryoid body formation excellent in formability of an embryoid body and suitable for optical observation.