Universal Antivenom Using Phage-Expressed Peptides for Multi-Species Venom

Solution Overview

Problem

Existing antibody-based antivenoms for snake bites are limited by high cost, side effects, and inefficacy against multiple venom types, requiring special storage conditions and being unsuitable for remote conditions.

Innovation Solution

Development of a multi-species antivenom composition using phage-expressed peptides that bind to conserved venom components, particularly targeting phospholipase A2, and a diagnostic kit for venom identification.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If antibody-based antivenom is used for snake bite treatment, then treatment effectiveness is achieved, but cost and side effects increase

Engineering Contradiction:

Improvetreatment effectivenessVSAvoidside effects

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent replaces expensive, complex antibody-based antivenom with inexpensive phage-displayed peptides that can be produced at low cost. These peptides are designed to be single-use or short-duration treatments that neutralize venom without the immunological side effects of heterologous antibodies, directly addressing the cost-side effect contradiction

Inventive Principle:

Principle #27Cheap short-living objects (Disposable)

Solution Approach 2:

The patent creates simplified copies of antibody binding sites using phage-displayed peptides. Instead of using full-length antibodies, the invention uses small peptide sequences (typically 15-50 amino acids) that replicate the essential venom-neutralizing function, reducing complexity and side effects while maintaining effectiveness

Inventive Principle:

Principle #26Copying

2Reliability

If antibody-based antivenom is used for snake bite treatment, then treatment effectiveness is achieved, but manufacturing cost increases

Engineering Contradiction:

Improvetreatment effectivenessVSAvoidmanufacturing cost

Core Design Contradiction:

ReliabilityVSEase of manufacture

Solution Approach 1:

The patent replaces expensive, complex antibody-based antivenom with inexpensive phage-displayed peptides that can be produced at low cost. These peptides are designed to be single-use or short-duration treatments that neutralize venom without the immunological side effects of heterologous antibodies, directly addressing the cost-side effect contradiction

Inventive Principle:

Principle #27Cheap short-living objects (Disposable)

Solution Approach 2:

The patent fundamentally changes the molecular parameters from large protein antibodies (150 kDa) to small peptides (2-10 kDa). This parameter change enables simpler production methods including chemical synthesis and phage display technology, dramatically reducing manufacturing costs while maintaining venom-neutralizing functionality

Inventive Principle:

Principle #35Parameter changes

3Reliability

If antibody-based antivenom is used for snake bite treatment, then treatment effectiveness is achieved, but storage requirements become complex

Engineering Contradiction:

Improvetreatment effectivenessVSAvoidstorage requirements

Core Design Contradiction:

ReliabilityVSEase of operation

Solution Approach 1:

The patent fundamentally changes the molecular parameters from large protein antibodies (150 kDa) to small peptides (2-10 kDa). This parameter change enables simpler production methods including chemical synthesis and phage display technology, dramatically reducing manufacturing costs while maintaining venom-neutralizing functionality

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The patent replaces expensive, complex antibody-based antivenom with inexpensive phage-displayed peptides that can be produced at low cost. These peptides are designed to be single-use or short-duration treatments that neutralize venom without the immunological side effects of heterologous antibodies, directly addressing the cost-side effect contradiction

Inventive Principle:

Principle #27Cheap short-living objects (Disposable)

4Reliability

If antibody-based antivenom is used for snake bite treatment, then treatment effectiveness is achieved, but applicability to multiple venom types is limited

Engineering Contradiction:

Improvetreatment effectivenessVSAvoidapplicability to multiple venom types

Core Design Contradiction:

ReliabilityVSAdaptability or versatility

Solution Approach 1:

The patent applies phage display technology to generate peptide libraries that can be screened against multiple venom types simultaneously. The resulting peptides are designed to recognize conserved epitopes across different snake species, creating universal antivenom that neutralizes multiple venom types with a single treatment, directly addressing the versatility limitation

Inventive Principle:

Principle #6Universality (Multi-functionality)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The phage-expressed peptides effectively neutralize venom toxicity across multiple species, providing a cost-effective and stable treatment option suitable for various venomous bites without special storage requirements.

Implementation Method 1

phage-expressed peptides that bind to conserved venom components, particularly targeting phospholipase A2

Methodology Applied

Scientific EffectMolecular binding: Adsorption

Data Source

PatentEP3582798B1Universal antivenom

Publication Date: 2025.07.23 DAYLIFE LLC

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AI summary

The present disclosure is directed to a universal antivenom for the treatment of venomous animal bites, and methods of developing the same using a novel targeted phage display technique.