Universal Nucleic Acid Purification Kit
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Solution Overview
Problem
Current methods for isolating RNA and DNA from biological samples are limited in versatility, often requiring separate kits and equipment, and do not allow for simultaneous extraction of both nucleic acids from the same sample, restricting users to specific stabilization products and purification options.
Innovation Solution
A method involving the use of Solubilization and Lysis Solutions with specific pH and amphiphilic reagents, followed by binding to a solid support, washing, and elution, which enables the extraction of either RNA or DNA, or both, from a single sample using a kit that includes Solubilization, Lysis, Binding, Wash, and Elution Solutions, allowing for flexible use with various collection tubes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If separate kits and equipment are used for RNA and DNA isolation, then purification reliability is improved, but device complexity and cost increase
Solution Approach 1:
The patent applies universality by developing a single purification kit that can isolate both RNA and DNA from the same sample using the same collection tube and purification protocol. The kit includes reagents and a protocol that enable users to obtain both nucleic acid types without requiring separate specialized kits, thereby reducing device complexity while maintaining purification reliability through optimized universal reagent formulations.
Solution Approach 2:
The patent merges the previously separate RNA and DNA isolation processes into a single unified protocol. By combining the purification steps and using a single collection tube that can yield both nucleic acids, the system reduces the number of separate operations required while ensuring reliable purification of both target molecules through integrated process design.
2Stability of the object's composition
If dedicated stabilization tubes are used for RNA or DNA, then nucleic acid stability is improved, but adaptability decreases
Solution Approach 1:
The patent applies universality by creating a single stabilization tube system that preserves both RNA and DNA simultaneously. The collection tube and stabilization reagents are designed to maintain the stability of both nucleic acid types without requiring separate dedicated tubes, thereby improving adaptability while maintaining nucleic acid stability through optimized stabilization chemistry.
3Manufacturing precision
If multiple separate kits are purchased for RNA and DNA isolation, then purification precision is improved, but loss of substance increases
Solution Approach 1:
The patent merges the isolation processes into a single protocol that extracts both RNA and DNA from the same sample in sequence. By using the same lysate and performing both purifications from one starting material, the system minimizes the loss of substance that would occur with separate processing while maintaining purification precision through optimized reagent formulations and protocols.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach provides a cost-effective and flexible method for isolating substantially pure and undegraded RNA or DNA, or both, from a single sample, overcoming the limitations of existing technologies by enabling simultaneous extraction and use with multiple collection tube manufacturers.
Implementation Method 1
contacting the crude lysate with a Solubilization Solution comprising a buffer at a pH between about 7 and 9, a base, an amphiphilic reagent
Implementation Method 2
contacting the sample with a Lysis Solution buffered at a pH of greater than about 7 to create an isolation sample, wherein the Lysis Solution comprises a complexing salt
Implementation Method 3
contacting the isolation sample to a solid support such that nucleic acids comprising substantially undegraded RNA in the isolation sample bind to the solid support
Data Source
AI summary
The invention features reagents, methods and kits for the purification of RNA, or DNA, or both, from a sample.