Universal Signal Peptide for Cross-Kingdom Protein Secretion
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Solution Overview
Problem
Current methods for producing recombinant proteins at an industrial scale face challenges in achieving efficient secretion across different host organisms, requiring multiple vectors and signal peptides specific to each host, which complicates the process and increases time and resources.
Innovation Solution
The use of a universal signal peptide sequence derived from Pseudogluconobacter saccharoketogenes PQQ-dependent alcohol dehydrogenase, which can direct the secretion of proteins in both prokaryotic and eukaryotic hosts, allowing for a single expression vector to be used across various organisms, simplifying the production process.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If multiple host-specific signal peptides and vectors are used for protein secretion, then protein secretion efficiency is improved for each host, but device complexity and time consumption increase
Solution Approach 1:
The patent applies universality by developing a single signal peptide sequence that can direct protein secretion across multiple host organisms (prokaryotes and eukaryotes). This universal signal peptide eliminates the need for host-specific signal peptides and vectors, thereby reducing device complexity while maintaining protein secretion efficiency across different hosts.
Solution Approach 2:
The patent merges the functions of multiple host-specific secretion systems into a single universal signal peptide sequence. By combining the secretion capabilities for prokaryotic and eukaryotic hosts into one unified signal peptide, the system reduces the number of separate vectors and signaling mechanisms needed, thus simplifying the overall production process.
2Reliability
If multiple host-specific vectors are used for protein expression, then expression specificity is improved, but loss of time and resources increase
Solution Approach 1:
The universal signal peptide sequence enables a single vector design to function across multiple host organisms. This universality eliminates the time-consuming process of preparing and optimizing separate vectors for each host, while maintaining expression specificity through the universal signal peptide's ability to recognize and direct secretion in diverse hosts.
Solution Approach 2:
The patent employs preliminary action by developing and validating the universal signal peptide sequence in advance. This pre-characterized signal peptide can be immediately applied to new protein expression projects across different hosts, eliminating the need for time-consuming signal peptide optimization and vector construction for each specific host- protein combination.
3Reliability
If host-specific signal peptides are used, then protein secretion reliability is improved for each host, but adaptability across different organisms decreases
Solution Approach 1:
The universal signal peptide sequence achieves universality by maintaining reliable protein secretion function across multiple host organisms including both prokaryotes and eukaryotes. This single signal peptide sequence can be applied to various host systems without requiring host-specific variants, thereby achieving both reliability in each host and adaptability across different organisms.
Data Source
AI summary
An amino acid sequence is described. The amino acid may comprise a signal sequence that is SEQ ID NO. 1a (or a variant or homologue or derivative or fragment thereof) that is expressed as a fusion protein that comprises a protein of interest. The signal sequence directs secretion of the protein of interest. The protein of interest may be a heterologous protein. Secretion of the fusion protein aids purification.