Urinalysis Kit Detecting Primary Cilia Components for Renal Disease Diagnosis
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Solution Overview
Problem
Current methods for diagnosing renal diseases are not rapid or simple, and there is a need for a kidney-specific and sensitive diagnostic technology that can detect renal injuries effectively.
Innovation Solution
A method and kit for urinalysis that detect primary cilia or components thereof, such as acetylated α-tubulin and ARL13B, isolated from renal tissues, using specific binding agents to identify their presence and concentration in urine samples, allowing for the diagnosis of renal diseases.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional diagnostic methods are used for renal diseases, then diagnosis can be performed, but the process is not rapid or simple and lacks kidney-specific sensitivity
Solution Approach 1:
The invention extracts primary cilia components (acetylated α-tubulin and ARL13B) specifically from renal tubular cells as diagnostic markers. By isolating and detecting these specific components in urine samples, the method achieves high kidney-specific sensitivity without requiring complex tissue biopsy procedures, thus resolving the contradiction between diagnostic precision and procedural complexity
Solution Approach 2:
The invention uses primary cilia components as intermediary markers that bridge the gap between renal tissue pathology and non-invasive urine analysis. These components serve as mediators that can be detected in urine to reflect renal tubular cell status, enabling simple urinalysis to achieve diagnostic sensitivity previously only attainable through complex tissue examination
2Speed
If primary cilia components are detected in urine, then rapid and specific renal disease diagnosis is achieved, but requires highly specific binding agents
Solution Approach 1:
The invention employs antibodies that can detect primary cilia components across multiple renal disease conditions (ischemia/reperfusion injury, acute kidney injury, chronic kidney disease). These multi-functional binding agents enable rapid diagnosis throughout the disease progression spectrum without requiring different reagents for different stages or types of renal injury, thus achieving speed without compromising detection capability
Solution Approach 2:
The invention detects changes in the presence, concentration, or structural state of primary cilia components (acetylated α-tubulin and ARL13B) in urine samples. By monitoring these parameter changes rather than requiring absolute quantification or complex structural analysis, the method achieves rapid diagnosis while using relatively simple detection techniques
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables rapid and specific diagnosis of renal diseases with high sensitivity and specificity using a small urine sample, capable of detecting primary cilia components in patients with various renal conditions, including ischemia/reperfusion injury, and monitoring disease progression.
Implementation Method 1
contacting the isolated urine sample with a binding agent that specifically binds to primary cilia or a component thereof isolated from renal tissues, or basal bodies of the primary cilia or a component thereof isolated from renal tissues
Data Source
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AI summary
The present invention relates to a composition for urinalysis for diagnosing a renal disease; a kit for urinalysis for diagnosing a renal disease; a method for detecting primary cilia or a component thereof isolated from renal tissues, or basal bodies of the primary cilia or a component thereof isolated from renal tissues; and a method for diagnosing the occurrence or progress of a renal disease. The composition and the kit for urinalysis of the present invention have the advantages of rapidly and simply identifying the presence of a renal disease by detecting the presence of primary cilia or a component thereof or proteins constituting the same from the urine of a renal disease patient.