Uterine Cervical Stem Cell Isolation Method
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Solution Overview
Problem
Current methods for isolating stem cells are invasive, painful, and yield low numbers, making it difficult to obtain clinically relevant amounts, especially from non-osteochondral mesodermal tissues like skin, fat, and muscle tissue.
Innovation Solution
A non-invasive and painless method for isolating stem cells from uterine cervix tissue, which involves preparing a cell suspension, recovering cells, incubating them in a suitable medium, and selecting the stem cells, allowing for the isolation of uterine cervical stem cells (UCESC) with enhanced anti-inflammatory, anti-tumor, and antimicrobial activities.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If stem cells are isolated from non-osteochondral mesodermal tissues like skin, fat, and muscle tissue using current methods, then the cells can be obtained for therapeutic applications, but the procedures are invasive, painful, and yield low numbers of cells
Solution Approach 1:
The patent extracts stem cells from a previously underutilized source (uterine cervix tissue) that can be obtained through routine gynecological procedures. By isolating stem cells from this accessible tissue source rather than requiring invasive procedures on muscle or fat tissue, the method achieves high cell yield without additional invasiveness or pain to the patient
Solution Approach 2:
The uterine cervix tissue naturally provides stem cells that can be harvested during routine gynecological examinations without requiring separate invasive procedures. The tissue itself serves as the source, and the routine examination procedure automatically provides access to the stem cells without additional harm or discomfort to the patient
2Adaptability or versatility
If stem cells are isolated from bone marrow or adipose tissue, then multipotent mesenchymal stromal cells can be obtained, but the harvest procedures are highly invasive
Solution Approach 1:
The patent demonstrates that uterine cervix stem cells possess universal multipotent characteristics similar to bone marrow and adipose-derived stem cells, capable of differentiating into multiple lineages. This universality allows the use of a less invasive tissue source (cervix) while maintaining the versatile therapeutic applications required for regenerative medicine
Solution Approach 2:
The uterine cervix serves as an intermediary tissue source that bridges the gap between easily accessible tissues and the traditional bone marrow/adipose sources. It provides stem cells with multipotent capabilities through a less invasive route, acting as a mediator that reduces procedural harm while maintaining cellular functionality
3Ease of operation
If stem cells are obtained from easily accessible tissues, then the isolation process is less invasive, but the number of cells obtained is insufficient for clinical applications
Solution Approach 1:
The patent performs preliminary enrichment of stem cells from uterine cervix tissue through a systematic isolation protocol that includes enzymatic digestion, density gradient centrifugation, and magnetic bead separation. This preliminary processing of the easily accessible cervical tissue concentrates the stem cells into a purified population suitable for clinical applications, overcoming the low yield problem
4Measurement precision
If stem cells are isolated using current phenotype characterization methods, then cell identity can be determined, but it is difficult to unequivocally distinguish between different multipotent cell types from soft tissue
Solution Approach 1:
The patent applies local quality by using a specific combination of surface markers (CD73, CD90, CD105 positivity with CD45, CD34, and HLA-DR negativity) that uniquely characterizes uterine cervix stem cells. This localized marker profile distinguishes these cells from other multipotent cell types in soft tissues, providing precise identification without requiring complex analytical systems
Data Source
AI summary
The present invention relates to a method for isolating stem cells comprising preparing a cell suspension from uterine cervix tissue, to the stem cells isolated by said method, and to the conditioned medium obtained from the culture of said stem cells. The invention also encompasses the use of said stem cells or conditioned medium for treating or preventing cancer, precancerous lesions, inflammatory diseases, autoimmune diseases, chronic pathologies or infectious diseases, diseases associated to tissue loss, or for use in diagnostic, prognostic or treatment of fertility disorders, as well as for cosmetic treatment.