Water-Soluble UV-Absorbing Polymer Dyes for Biological Detection
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Solution Overview
Problem
Existing UV-absorbing polymers are often hydrophobic, leading to poor solubility and limited utility in aqueous conditions, which restricts their application in biological detection methods due to broadened spectra and reduced brightness.
Innovation Solution
Development of novel UV-excitable polymer dyes and conjugates with specific structural formulations, including water-solubilizing moieties, that absorb in the near ultraviolet range, enhancing solubility and specificity for biological applications.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If UV-absorbing polymers are used for biological detection, then they can generate signals for real-time monitoring, but they exhibit poor water solubility and broadened spectra
Solution Approach 1:
The patent modifies the chemical structure of UV-absorbing polymers by incorporating water-solubilizing groups (such as sulfonate, carboxylate, ammonium, and hydroxyl groups) to change the solubility parameter from hydrophobic to hydrophilic, enabling aqueous applications while maintaining UV absorption and fluorescence properties
Solution Approach 2:
The patent creates composite polymer structures combining UV-absorbing chromophores with water-solubilizing moieties and linker groups, forming a multi-component molecular architecture that simultaneously provides UV absorption, water solubility, and biological conjugation capability
2Productivity
If UV-absorbing polymers are used for biological detection, then they can provide rapid detection methods, but they show reduced brightness and broadened spectra
Solution Approach 1:
The patent optimizes the chemical structure to narrow the absorption spectra and enhance fluorescence quantum yield, achieving sharper spectral peaks and increased brightness through precise control of chromophore structure and solvent interactions
3Ease of manufacture
If hydrophobic UV-absorbing polymers are used, then they can be synthesized with UV absorption properties, but they are not useful under aqueous conditions
Solution Approach 1:
The patent changes the hydrophobicity parameter of the polymer by incorporating polar and ionizable groups, transforming the material from hydrophobic to hydrophilic to enable aqueous solubility while preserving UV absorption and fluorescence properties
Solution Approach 2:
The patent designs polymers with multiple functional groups that provide simultaneous UV absorption, water solubility, and conjugation capability, making the polymer universally applicable to various biological detection scenarios
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The novel UV-absorbing polymers demonstrate improved water solubility and spectral specificity, enabling effective detection of analytes in biological samples with reduced non-specific interactions and enhanced signal-to-noise ratios in flow cytometry.
Implementation Method 1
UV-absorbing polymers that absorb in the near ultraviolet range
Implementation Method 2
generating signals which can be monitored in real time
Data Source
AI summary
The present disclosure provides UV-absorbing polymer dyes and methods for detecting an analyte in a sample by using a binding partner conjugated to a UV-absorbing polymer dye. Compositions comprising UV-absorbing polymer dyes, UV-absorbing tandem dyes, or quenched UV polymer dyes are provided.