UV Fluorescence Spectral Collection Within Detector Response Range
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Solution Overview
Problem
Existing fluorescence analysis methods face challenges in keeping the peaks of spectral data within the recognizable range of imaging spectral detection devices, leading to inaccurate analysis results due to wide wavelength ranges of fluorescence radiation signals.
Innovation Solution
A method and system for collecting fluorescence spectral data using ultraviolet light within a specific wavelength range (300-390 nm) followed by imaging and calculating spectral data within 400-800 nm, utilizing a spectrum reconstruction algorithm to ensure peak values fall within the effective response range of the detection device.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If fluorescence radiation signal with wide wavelength range is used, then high sensitivity and strong selectivity are achieved, but peak values may fall beyond the recognizable range of the spectral detection apparatus
Solution Approach 1:
The patent changes the excitation light wavelength parameter from conventional ultraviolet ranges to specifically 300-390nm, which shifts the fluorescence emission peaks into the 400-800nm detectable range of imaging spectral detection devices while maintaining the high sensitivity and selectivity of fluorescence analysis
Solution Approach 2:
The patent transitions from traditional point-by-point spectral detection to imaging spectral detection, adding spatial dimensionality to the measurement. This allows simultaneous acquisition of spectral data across multiple locations, ensuring peaks fall within the recognizable range while maintaining analytical capability
2Power
If conventional ultraviolet light is used for excitation, then fluorescence signals are generated, but peaks of spectral data fall beyond the recognizable range of the imaging spectral detection device
Solution Approach 1:
The patent modifies the excitation wavelength parameter to 300-390nm ultraviolet range, which generates strong fluorescence signals while positioning the emission peaks within the 400-800nm detectable range of imaging spectral detection devices, simultaneously achieving high signal intensity and accurate peak positioning
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for accurate and non-invasive testing of analytes by keeping spectral data peaks within the recognizable range, enabling low-cost, miniaturized, and real-time analysis without the need for electrochemical reactions.
Implementation Method 1
A fluorescence analysis method means a method for qualitative or quantitative analysis of fluorescence that can reflect characteristics of some substances and that is produced during a process in which the substances are in an excited state after being irradiated with ultraviolet light
Implementation Method 2
a spectral detection apparatus, wherein the spectral detection apparatus is an imaging spectral detection apparatus with an effective response range of 400-800 nm
Data Source
AI summary
The present invention provides a method and system for collecting fluorescence spectral data by ultraviolet light, and a medium. The method includes: ultraviolet light irradiation: irradiating a first area by the ultraviolet light with a wavelength of 300-390 nm; signal collection: obtaining a fluorescence radiation signal that comprises the fluorescence spectral data and that is emitted by the first area when excited, and performing imaging, to obtain an image; and spectral data collection: selecting a data collection point from the image, substituting a grayscale value of the data collection point into a spectrum reconstruction algorithm, and obtaining the fluorescence spectral data through calculation, where a peak value of a spectral line of the fluorescence spectral data is within 400-800 nm. According to the technical solution in this application, peaks of spectral data of different analytes can be kept within a recognizable range of an imaging spectral detection apparatus.