UV Fluorescence Spectral Reconstruction Within Detector Range
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Solution Overview
Problem
Existing fluorescence analysis methods face challenges in keeping the peaks of spectral data within the recognizable range of imaging spectral detection devices, leading to inaccurate analysis results due to wide wavelength ranges of fluorescence radiation signals.
Innovation Solution
A method and system utilizing ultraviolet light irradiation in the 300-390 nm range to excite fluorescence spectral data, followed by image processing to select testing and reference points within the 400-800 nm range for accurate spectral data collection, using a spectrum reconstruction algorithm to ensure peak values fall within the detection device's effective range.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If fluorescence radiation signal with wide wavelength range is used, then high sensitivity and strong selectivity are achieved, but peak values may fall beyond the recognizable range of the spectral detection apparatus
Solution Approach 1:
The patent applies parameter changes by selecting specific excitation wavelength ranges (380-450nm, 450-550nm, 550-650nm, 650-780nm) to control the fluorescence emission peaks within the detectable range of the imaging spectral detection device. By adjusting the excitation parameters, the system ensures that different analytes produce spectral peaks that fall within the recognizable range of the detector, resolving the contradiction between maintaining high sensitivity and ensuring detectability.
2Measurement precision
If multiple sensors and modules are used to collect biological signals from different positions, then comprehensive analysis is achieved, but system complexity and cost increase
Solution Approach 1:
The patent implements a universal imaging spectral detection device that can collect fluorescence spectral data from multiple analytes simultaneously using a single integrated system. The device uses broadband excitation light sources and spectral filtering to detect multiple analytes (glucose, cholesterol, uric acid, etc.) at different wavelengths within the visible range, eliminating the need for multiple separate sensors and modules while maintaining comprehensive analysis capability.
Solution Approach 2:
The patent combines multiple detection functions into a single imaging spectral detection device that integrates excitation light sources, spectral filters, and detection sensors. By merging the collection of fluorescence signals from different analytes into one unified system, the patent reduces system complexity and cost while maintaining the ability to perform comprehensive biochemical analysis.
3Ease of operation
If absorption spectroscopy is used for non-invasive testing, then non-invasive measurement is achieved, but spectral signals include interference from skin tissue and other components
Solution Approach 1:
The patent uses fluorescence emission as an intermediary signal that occurs at longer wavelengths than the excitation light. By exciting analytes with ultraviolet or visible light and detecting the resulting fluorescence emission in the visible range, the system achieves non-invasive measurement while obtaining spectral signals that are less affected by skin tissue absorption and scattering, thereby improving signal purity compared to direct absorption spectroscopy.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for non-invasive, cost-effective, and real-time testing by maintaining spectral data peaks within the detection device's range, enabling accurate analysis of analytes like glucose without invasive procedures and reducing interference from non-analyte components.
Implementation Method 1
irradiating a first area by the ultraviolet light with a wavelength of 300-390 nm; obtaining a fluorescence radiation signal that includes fluorescence spectral data and that is emitted by the first area when excited
Data Source
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AI summary
The present invention provides a method and system for collecting fluorescence spectral data by ultraviolet light, and a medium. The method includes: ultraviolet light irradiation: irradiating a first area by the ultraviolet light with a wavelength of 300-390 nm; signal collection: obtaining a fluorescence radiation signal that comprises the fluorescence spectral data and that is emitted by the first area when excited, and performing imaging, to obtain an image; and spectral data collection: selecting a data collection point from the image, substituting a grayscale value of the data collection point into a spectrum reconstruction algorithm, and obtaining the fluorescence spectral data through calculation, where a peak value of a spectral line of the fluorescence spectral data is within 400-800 nm. According to the technical solution in this application, peaks of spectral data of different analytes can be kept within a recognizable range of an imaging spectral detection apparatus.