UV-Grafted COP Microfluidic Surfaces for Biofouling Resistance

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Solution Overview

Problem

The surface modification of cyclic olefin polymer (COP) and cyclic olefin copolymer (COC) substrates used in microfluidic devices is challenging due to their non-reactivity, leading to inconsistent performance and biofouling issues, as existing methods like silane modification result in non-covalent attachments and UV-graft polymerization techniques are not widely adopted.

Innovation Solution

A covalently modified polymer surface is achieved using a graft copolymer comprising a backbone polymer, a polyalkylene glycol acrylic side chain, and a fluorinated acrylic side chain, which is produced through UV-graft polymerization with a benzophenone photoinitiator, resulting in a surface with a high water contact angle and resistance to biofouling.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If silane modification is used to improve microfluidic device surface tensions, then surface tension properties can be altered, but the surface modification produces inconsistent quality and performance due to non-covalent attachment

Engineering Contradiction:
Improvesurface modification consistencyVSAvoidcovalent attachment strength
Core Design Contradiction:
ReliabilityVSStability of the object's composition

Solution Approach 1:

The invention changes the chemical parameter of the surface modification process by using UV-initiated graft polymerization instead of silane chemistry. This creates covalent C-C bonds between the copolymer and the COP/COC substrate, fundamentally changing the attachment strength and consistency parameters to achieve reliable, reproducible surface properties.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The invention creates a composite structure by grafting a copolymer containing both hydrophobic and non-biofouling moieties onto the substrate. This composite material combines the desirable properties of hydrophobicity with resistance to protein adsorption, achieving both high water contact angle and biofouling resistance simultaneously.

Inventive Principle:
Principle #40Composite materials

2Ease of operation

If the surface is made highly hydrophobic to manipulate aqueous droplets, then droplet manipulation improves, but the surface becomes more susceptible to biofouling and protein accumulation

Engineering Contradiction:
Improvedroplet manipulation capabilityVSAvoidbiofouling susceptibility
Core Design Contradiction:
Ease of operationVSObject-affected harmful factors

Solution Approach 1:

The invention applies local quality by incorporating different functional moieties at different locations within the copolymer structure. The copolymer contains both hydrophobic groups (for droplet manipulation) and non-biofouling groups (for protein resistance) distributed throughout the graft chains, allowing both functions to coexist on the same surface.

Inventive Principle:
Principle #3Local quality

Solution Approach 2:

The invention changes the surface chemistry parameter by introducing a copolymer with dual functionality. The copolymer composition is specifically designed to balance hydrophobicity (for droplet handling) and non-biofouling properties (for protein resistance), achieving a unique surface property profile that simultaneously enables both droplet manipulation and biofouling resistance.

Inventive Principle:
Principle #35Parameter changes

3Reliability

If UV-graft polymerization is used to covalently modify the surface, then surface robustness improves, but the process complexity increases compared to silane modification

Engineering Contradiction:
Improvesurface attachment robustnessVSAvoidmodification process complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The invention applies preliminary action by pre-synthesizing the copolymer with the desired composition and properties before the grafting step. This eliminates the need for complex in-situ polymerization control and allows the UV-grafting process to focus solely on attaching the pre-formed copolymer to the substrate, simplifying the overall process while maintaining covalent attachment robustness.

Inventive Principle:
Principle #10Preliminary action

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The modified surface maintains excellent hydrophobic characteristics and resistance to biofouling even after exposure to protein solutions, with a water contact angle change of less than 10°, ensuring robust and consistent performance.

Implementation Method 1

The use of a benzophenone photoinitiator in the presence of a solution phase of monomer allows COP and COC substrates to be covalently modified

Methodology Applied
Scientific EffectPhotopolymerization: Photopolymerisation

Implementation Method 2

the microfluidic surfaces be not only hydrophobic, but also non-biofouling

Methodology Applied
Scientific EffectHydrophobic effect: Hydrophobe

Data Source

PatentEP3558527B1UV graft on microfluidic devices
Publication Date: 2023.05.03 BIO RAD LABORATORIES INC
  • EP3558527B1 patent drawingFigure 1
  • EP3558527B1 patent drawingFigure 2
  • EP3558527B1 patent drawingFigure 3

AI summary

Compositions, devices, and methods are disclosed for the covalent modification of polymer surfaces with graft copolymers having a blend of side chains. The halogenated acrylic and polyalkylene glycol acrylic side chains of the graft copolymer provide the polymer surface with high hydrophobicity, as well as increased resistance to biofouling with proteinaceous material. The polymer surfaces can be particularly useful in microfluidic devices and methods that involve the contacting of the covalently modified polymer surfaces with emulsions of aqueous droplets containing biological macromolecules within an oil carrier phase.