UV-LED Well Plate Induction for Residue-Free Cell Stress

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Solution Overview

Problem

Existing stress induction systems are bulky, non-portable, and utilize outdated techniques, such as radiation-based or chemical induction, which are messy and leave residues, making them unsuitable for precise biological assays.

Innovation Solution

A portable UV-LED-based optogenetic system that induces stress in cells instantaneously and can be turned on/off in milliseconds, using a well plate with a light cone array and UV LEDs, controlled by an algorithm to modulate wavelengths and exposure.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If radiation-based systems or chemical induction are used for stress induction, then biological endpoints can be induced, but the systems become bulky, non-portable, and leave residues on cells

Engineering Contradiction:

Improvebiological endpoint inductionVSAvoidportability

Core Design Contradiction:

ReliabilityVSEase of operation

Solution Approach 1:

The patent replaces bulky radiation-based systems and chemical induction methods with a portable UV-LED array system. The UV-LEDs emit specific wavelengths (280-315 nm) to induce stress in cells without requiring large equipment or leaving chemical residues, enabling precise control and portability while maintaining reliable biological endpoint induction

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent changes the induction method from chemical or broad-spectrum radiation to targeted UV-LED wavelengths (280-315 nm). This parameter change allows selective induction of biological endpoints with precise spatial and temporal control, eliminating residues and reducing system size while maintaining induction effectiveness

Inventive Principle:

Principle #35Parameter changes

2Reliability

If chemical induction is used, then stress can be induced in cells, but the process is messy and leaves residue requiring multiple washing steps

Engineering Contradiction:

Improvestress inductionVSAvoidchemical residue

Core Design Contradiction:

ReliabilityVSLoss of substance

Solution Approach 1:

The patent substitutes chemical induction with physical UV-LED light induction. The UV-LEDs directly induce stress responses in cells through photobiological effects without introducing chemical substances, thereby eliminating the need for washing steps and preventing chemical residue contamination that would interfere with subsequent assays

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent extracts and eliminates the chemical induction step entirely, replacing it with direct UV-LED irradiation. This removes the source of chemical residues from the system, allowing cells to be induced and analyzed without messy chemical handling or extensive washing protocols

Inventive Principle:

Principle #2Taking out (Extraction)

3Reliability

If chemical induction is used, then stress can be induced, but the process requires incubation and multiple washing steps which is time-consuming

Engineering Contradiction:

Improvestress inductionVSAvoidinduction process time

Core Design Contradiction:

ReliabilityVSLoss of time

Solution Approach 1:

The patent replaces the multi-step chemical induction process (application, incubation, washing) with instantaneous UV-LED irradiation. The UV-LEDs can be turned on and off in milliseconds to induce stress directly in cells, eliminating incubation and washing steps while maintaining reliable stress induction, thereby dramatically reducing total process time

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

4Ease of operation

If UV-LED array is used for induction, then portability and precision are improved, but device complexity increases

Engineering Contradiction:

ImproveportabilityVSAvoidsystem structure

Core Design Contradiction:

Ease of operationVSDevice complexity

Solution Approach 1:

The patent divides the induction system into modular UV-LED units arranged in arrays, each emitting specific wavelengths. This segmentation allows the system to be configured in portable formats while maintaining precise spectral control, balancing portability with the complexity needed for targeted biological endpoint induction

Inventive Principle:

Principle #1Segmentation

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Enables precise, residue-free, and efficient induction of measurable biological endpoints in small batches, suitable for portable use and further analysis.

Implementation Method 1

utilizing new light emitting diodes allows for more portable and smaller batch applications

Methodology Applied

Scientific EffectLight Emitting Diode: Light Emitting Diode

Implementation Method 2

a damage induction system, utilizing a light emitting diode, in conjunction with a specimen well plate

Methodology Applied

Scientific EffectUltraviolet radiation: Absorption (EM radiation)

Data Source

PatentUS12502549B2UV-LED induction of measurable biological endpoints

Publication Date: 2025.12.23 AMELIA TECH

AI summary

Composition, systems, and methods for the induction of biological endpoints in a cheap contained system. In many embodiments, variation on well numbers on plates and ranging ultraviolet wavelengths produces different and controlled outcomes of the biological endpoint induction. Endpoint induction of this type allows for chemical induction, wherein the system can be turned on and off in short time periods.